Comparative field performance over 3 years and two sites of transgenic wheat lines expressing HMW subunit transgenes

Peter R. Shewry, Stephen Powers, J. Michael Field, Roger J Fido, Huw D Jones, Gillian M Arnold, Jevon West, Paul A. Lazzeri, Pilar Barcelo, Francisco Barro, Arthur S. Tatham, Frank Bekes, Barbara Butow, Helen Darlington

Research output: Contribution to journalArticlepeer-review

48 Citations (SciVal)

Abstract

A series of transgenic wheat lines expressing additional high molecular weight (HMW) subunit genes and the corresponding control lines were grown in replicate field trials at two UK sites (Rothamsted Research, approximately 50 km north of London and Long Ashton, near Bristol) over 3 years (1998, 1999, 2000), with successive generations of the transgenic lines (T3, T4, T5) being planted. Four plots from each site were used to determine grain dry weight, grain nitrogen, dough strength (measured as peak resistance by Mixograph analysis) and the expression levels of the endogenous and "added" subunits. Detailed statistical analyses showed that the transgenic and non-transgenic lines did not differ in terms of stability of HMW subunit gene expression or in stability of grain nitrogen, dry weight or dough strength, either between the 3 years or between sites and plots. These results indicate that the transgenic and control lines can be regarded as substantially equivalent in terms of stability of gene expression between generations and environments.

Original languageEnglish
Pages (from-to)128-136
Number of pages9
JournalTheoretical and Applied Genetics
Volume113
Issue number1
DOIs
Publication statusPublished - Jun 2006

Keywords

  • Breeding
  • Gene Expression
  • Genomic Instability
  • Glutens
  • Molecular Weight
  • Plants, Genetically Modified
  • Protein Subunits
  • Seeds
  • Triticum

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