Comparative genomic analysis of six Glossina genomes, vectors of African trypanosomes

Geoffrey M. Attardo, Adly M. M. Abd-Alla, Alvaro Acosta-Serrano, James E. Allen, Rosemary Bateta, Joshua B. Benoit, Kostas Bourtzis, Jelle Caers, Guy Caljon, Mikkel B. Christensen, David W. Farrow, Markus Friedrich, Aurélie Hua-Van, Emily C. Jennings, Denis M. Larkin, Daniel Lawson, Michael J. Lehane, Vasileios P. Lenis, Ernesto Lowy-Gallego, Rosaline W. MachariaAnna R. Malacrida, Heather G. Marco, Daniel Masiga, Gareth L. Maslen, Irina Matetovici, Richard P. Meisel, Irene Meki, Veronika Michalkova, Wolfgang J. Miller, Patrick Minx, Paul O. Mireji, Lino Ometto, Andrew G. Parker, Rita Rio, Clair Rose, Andrew J. Rosendale, Omar Rota-Stabelli, Grazia Savini, Liliane Schoofs, Francesca Scolari, Martin T. Swain, Peter Takáč, Chad Tomlinson, George Tsiamis, Jan Van Den Abbeele, Aurelien Vigneron, Jingwen Wang, Wesley C. Warren, Robert M. Waterhouse, Matthew T. Weirauch, Brian L. Weiss, Richard K. Wilson, Xin Zhao, Serap Aksoy

Research output: Contribution to journalArticlepeer-review

45 Citations (SciVal)
151 Downloads (Pure)

Abstract

Background
Tsetse flies (Glossina sp.) are the vectors of human and animal trypanosomiasis throughout sub-Saharan Africa. Tsetse flies are distinguished from other Diptera by unique adaptations, including lactation and the birthing of live young (obligate viviparity), a vertebrate blood-specific diet by both sexes, and obligate bacterial symbiosis. This work describes the comparative analysis of six Glossina genomes representing three sub-genera: Morsitans (G. morsitans morsitans, G. pallidipes, G. austeni), Palpalis (G. palpalis, G. fuscipes), and Fusca (G. brevipalpis) which represent different habitats, host preferences, and vectorial capacity.

Results
Genomic analyses validate established evolutionary relationships and sub-genera. Syntenic analysis of Glossina relative to Drosophila melanogaster shows reduced structural conservation across the sex-linked X chromosome. Sex-linked scaffolds show increased rates of female-specific gene expression and lower evolutionary rates relative to autosome associated genes. Tsetse-specific genes are enriched in protease, odorant-binding, and helicase activities. Lactation-associated genes are conserved across all Glossina species while male seminal proteins are rapidly evolving. Olfactory and gustatory genes are reduced across the genus relative to other insects. Vision-associated Rhodopsin genes show conservation of motion detection/tracking functions and variance in the Rhodopsin detecting colors in the blue wavelength ranges.

Conclusions
Expanded genomic discoveries reveal the genetics underlying Glossina biology and provide a rich body of knowledge for basic science and disease control. They also provide insight into the evolutionary biology underlying novel adaptations and are relevant to applied aspects of vector control such as trap design and discovery of novel pest and disease control strategies.
Original languageEnglish
Article number187
Number of pages31
JournalGenome Biology
Volume20
Issue number1
DOIs
Publication statusPublished - 02 Sept 2019

Keywords

  • Disease
  • Hematophagy
  • Lactation
  • Neglected
  • Symbiosis
  • Trypanosomiasis
  • Tsetse
  • Geography
  • Genomics
  • Wolbachia/genetics
  • Male
  • Insect Proteins/genetics
  • Phylogeny
  • Repetitive Sequences, Nucleic Acid/genetics
  • Genes, X-Linked
  • Trypanosoma/parasitology
  • Female
  • Genome, Insect
  • Genes, Insect
  • Tsetse Flies/genetics
  • DNA Transposable Elements/genetics
  • Gene Expression Regulation
  • Drosophila melanogaster/genetics
  • Sequence Homology, Amino Acid
  • Mutagenesis, Insertional/genetics
  • Animals
  • Synteny/genetics
  • Insect Vectors/genetics

Fingerprint

Dive into the research topics of 'Comparative genomic analysis of six Glossina genomes, vectors of African trypanosomes'. Together they form a unique fingerprint.

Cite this