Comparison of PCR-based marker systems for the analysis of genetic relationships in cultivated potato

Dan Milbourne, Rhonda Meyer, John E. Bradshaw, Eileen Baird, Nicky Bonar, Jim Provan, Wayne Powell, Robbie Waugh

Research output: Contribution to journalReview articlepeer-review

357 Citations (SciVal)

Abstract

The application of AFLPs, RAPDs and SSRs to examine genetic relationships in the primary northwestern European cultivated potato gene pool was investigated. Sixteen potato cultivars were genotyped using five AFLP primer combinations, 14 RAPD primers, and 17 database-derived SSR primer pairs. All three approaches successfully discriminated between the 16 cultivars using a minimum of one assay. Similarity matrices produced for each marker type on the basis of Nei and Li coefficients showed low correlations when compared with different statistical tests. Dendrograms were produced from these data for each marker system. The usefulness of each system was examined in terms of number of loci revealed (effective multiplex ratio, or EMR) and the amount of polymorphism detected (diversity index, or DI). AFLPs had the highest EMR, and SSRs the highest DI. A single parameter, marker index (MI), which is the product of DI and EMR, was used to evaluate the overall utility of each marker system. The use of these PCR-based marker systems in potato improvement and statutory applications is discussed.

Original languageEnglish
Pages (from-to)127-136
Number of pages10
JournalMolecular Breeding
Volume3
Issue number2
DOIs
Publication statusPublished - Apr 1997

Keywords

  • AFLP
  • genetic relationships
  • potato
  • RAPD
  • SSR
  • FRAGMENT LENGTH POLYMORPHISMS
  • RAPD MARKERS
  • SOLANUM-TUBEROSUM
  • DNA
  • RFLP
  • MICROSATELLITES
  • SEQUENCE
  • LINKAGE
  • FINGERPRINTS
  • ABUNDANCE
  • Genetic relationships
  • Potato

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