Comparison of ribosomal DNA sites inLolium species by fluorescencein situ hybridization

H. M. Thomas; J. A. Harper; M. R. Meredith; W. G. Morgan; Ian Thomas; Emma Timms-Taravella; Ian  King

doi:10.1007/BF02261775

Comparison of ribosomal DNA sites inLolium species by fluorescencein situ hybridization

H. M. Thomas, J. A. Harper, M. R. Meredith, W. G. Morgan, Ian Thomas, Emma Timms-Taravella, Ian King

Department of Life Sciences

Institute of Grassland and Environmental Research

Research output: Contribution to journal › Article › peer-review

64 Citations (SciVal)

Abstract

The position of the 18S-5.8S-26S and 5S rRNA genes have been physically mapped on the chromosomes of sevenLolium taxa. 18S-5.8S-26S sites were seen on two pairs of chromosomes in the inbreeding taxa. In the outbreeding taxa six sites were found in theL. multiflorum, seven inL. perenne and nine inL. rigidum var.rigidum. Two 5S sites were found in each of the taxa. In the inbreeders, the 5S sites were found adjacent to the 18S-5.8S-26S sites on chromosome 2. InL. multiflorum andL. perenne the 5S sites were on the short arm of chromosome 3. However, inL. rigidum var.rigidum the 5S rDNA site was found in either of the two positions.

Original language	English
Pages (from-to)	486-490
Number of pages	5
Journal	Chromosome Research
Volume	4
Issue number	7
DOIs	https://doi.org/10.1007/BF02261775
Publication status	Published - Nov 1996

Keywords

in situ hybridization
Lolium
phylogeny
rDNA

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title = "Comparison of ribosomal DNA sites inLolium species by fluorescencein situ hybridization",

abstract = "The position of the 18S-5.8S-26S and 5S rRNA genes have been physically mapped on the chromosomes of sevenLolium taxa. 18S-5.8S-26S sites were seen on two pairs of chromosomes in the inbreeding taxa. In the outbreeding taxa six sites were found in theL. multiflorum, seven inL. perenne and nine inL. rigidum var.rigidum. Two 5S sites were found in each of the taxa. In the inbreeders, the 5S sites were found adjacent to the 18S-5.8S-26S sites on chromosome 2. InL. multiflorum andL. perenne the 5S sites were on the short arm of chromosome 3. However, inL. rigidum var.rigidum the 5S rDNA site was found in either of the two positions.",

keywords = "in situ hybridization, Lolium , phylogeny, rDNA",

author = "Thomas, {H. M.} and Harper, {J. A.} and Meredith, {M. R.} and Morgan, {W. G.} and Ian Thomas and Emma Timms-Taravella and Ian King",

year = "1996",

month = nov,

doi = "10.1007/BF02261775",

language = "English",

volume = "4",

pages = "486--490",

journal = "Chromosome Research",

issn = "0967-3849",

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TY - JOUR

T1 - Comparison of ribosomal DNA sites inLolium species by fluorescencein situ hybridization

AU - Thomas, H. M.

AU - Harper, J. A.

AU - Meredith, M. R.

AU - Morgan, W. G.

AU - Thomas, Ian

AU - Timms-Taravella, Emma

AU - King, Ian

PY - 1996/11

Y1 - 1996/11

N2 - The position of the 18S-5.8S-26S and 5S rRNA genes have been physically mapped on the chromosomes of sevenLolium taxa. 18S-5.8S-26S sites were seen on two pairs of chromosomes in the inbreeding taxa. In the outbreeding taxa six sites were found in theL. multiflorum, seven inL. perenne and nine inL. rigidum var.rigidum. Two 5S sites were found in each of the taxa. In the inbreeders, the 5S sites were found adjacent to the 18S-5.8S-26S sites on chromosome 2. InL. multiflorum andL. perenne the 5S sites were on the short arm of chromosome 3. However, inL. rigidum var.rigidum the 5S rDNA site was found in either of the two positions.

AB - The position of the 18S-5.8S-26S and 5S rRNA genes have been physically mapped on the chromosomes of sevenLolium taxa. 18S-5.8S-26S sites were seen on two pairs of chromosomes in the inbreeding taxa. In the outbreeding taxa six sites were found in theL. multiflorum, seven inL. perenne and nine inL. rigidum var.rigidum. Two 5S sites were found in each of the taxa. In the inbreeders, the 5S sites were found adjacent to the 18S-5.8S-26S sites on chromosome 2. InL. multiflorum andL. perenne the 5S sites were on the short arm of chromosome 3. However, inL. rigidum var.rigidum the 5S rDNA site was found in either of the two positions.

KW - in situ hybridization

KW - Lolium

KW - phylogeny

KW - rDNA

UR - http://hdl.handle.net/2160/44831

U2 - 10.1007/BF02261775

DO - 10.1007/BF02261775

M3 - Article

SN - 0967-3849

VL - 4

SP - 486

EP - 490

JO - Chromosome Research

JF - Chromosome Research

IS - 7

ER -

Comparison of ribosomal DNA sites inLolium species by fluorescencein situ hybridization

Abstract

Keywords

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