Comparison of tuberculin activity using the interferon-γ assay for the diagnosis of bovine tuberculosis

I. Schiller, H. M. Vordermeier, W. R. Waters, A. Kyburz, M. Cagiola, A. Whelan, M. V. Palmer, T. C. Thacker, J. Meijlis, C. Carter, S. Gordon, T. Egnuni, R. Hardegger, B. Marg-Haufe, A. Raeber, B. Oesch*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

26 Citations (SciVal)

Abstract

In this study, interferon-γ (IFN-γ) responses in whole blood cultures stimulated with tuberculins from different sources were compared with regard to their diagnostic reliability in cattle experimentally and naturally infected with Mycobacterium bovis. The IFN-γ responses to different concentrations of purified protein derivatives (PPDs) from M bovis and Mycobacterium avium were quantified. Significant differences (P<0.05) between sources and concentrations of PPDs used for stimulation were detected, indicating a need for standardisation of PPDs used in the IFN-γ assay. Additionally, a tool named 'relative potency 30' that allows rapid comparison of batches and sources of PPDs was defined.

Original languageEnglish
Pages (from-to)322-326
Number of pages5
JournalVeterinary Record
Volume167
Issue number9
DOIs
Publication statusPublished - 28 Aug 2010

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