Comparison of tuberculin activity using the interferon-γ assay for the diagnosis of bovine tuberculosis

I. Schiller; H. M. Vordermeier; W. R. Waters; A. Kyburz; M. Cagiola; A. Whelan; M. V. Palmer; T. C. Thacker; J. Meijlis; C. Carter; S. Gordon; T. Egnuni; R. Hardegger; B. Marg-Haufe; A. Raeber; B. Oesch

doi:10.1136/vr.c3403

Comparison of tuberculin activity using the interferon-γ assay for the diagnosis of bovine tuberculosis

I. Schiller, H. M. Vordermeier, W. R. Waters, A. Kyburz, M. Cagiola, A. Whelan, M. V. Palmer, T. C. Thacker, J. Meijlis, C. Carter, S. Gordon, T. Egnuni, R. Hardegger, B. Marg-Haufe, A. Raeber, B. Oesch^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

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Abstract

In this study, interferon-γ (IFN-γ) responses in whole blood cultures stimulated with tuberculins from different sources were compared with regard to their diagnostic reliability in cattle experimentally and naturally infected with Mycobacterium bovis. The IFN-γ responses to different concentrations of purified protein derivatives (PPDs) from M bovis and Mycobacterium avium were quantified. Significant differences (P<0.05) between sources and concentrations of PPDs used for stimulation were detected, indicating a need for standardisation of PPDs used in the IFN-γ assay. Additionally, a tool named 'relative potency 30' that allows rapid comparison of batches and sources of PPDs was defined.

Original language	English
Pages (from-to)	322-326
Number of pages	5
Journal	Veterinary Record
Volume	167
Issue number	9
DOIs	https://doi.org/10.1136/vr.c3403
Publication status	Published - 28 Aug 2010

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Schiller, I., Vordermeier, H. M., Waters, W. R., Kyburz, A., Cagiola, M., Whelan, A., Palmer, M. V., Thacker, T. C., Meijlis, J., Carter, C., Gordon, S., Egnuni, T., Hardegger, R., Marg-Haufe, B., Raeber, A., & Oesch, B. (2010). Comparison of tuberculin activity using the interferon-γ assay for the diagnosis of bovine tuberculosis. Veterinary Record, 167(9), 322-326. https://doi.org/10.1136/vr.c3403

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abstract = "In this study, interferon-γ (IFN-γ) responses in whole blood cultures stimulated with tuberculins from different sources were compared with regard to their diagnostic reliability in cattle experimentally and naturally infected with Mycobacterium bovis. The IFN-γ responses to different concentrations of purified protein derivatives (PPDs) from M bovis and Mycobacterium avium were quantified. Significant differences (P<0.05) between sources and concentrations of PPDs used for stimulation were detected, indicating a need for standardisation of PPDs used in the IFN-γ assay. Additionally, a tool named 'relative potency 30' that allows rapid comparison of batches and sources of PPDs was defined.",

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Schiller, I, Vordermeier, HM, Waters, WR, Kyburz, A, Cagiola, M, Whelan, A, Palmer, MV, Thacker, TC, Meijlis, J, Carter, C, Gordon, S, Egnuni, T, Hardegger, R, Marg-Haufe, B, Raeber, A & Oesch, B 2010, 'Comparison of tuberculin activity using the interferon-γ assay for the diagnosis of bovine tuberculosis', Veterinary Record, vol. 167, no. 9, pp. 322-326. https://doi.org/10.1136/vr.c3403

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AU - Schiller, I.

AU - Vordermeier, H. M.

AU - Waters, W. R.

AU - Kyburz, A.

AU - Cagiola, M.

AU - Whelan, A.

AU - Palmer, M. V.

AU - Thacker, T. C.

AU - Meijlis, J.

AU - Carter, C.

AU - Gordon, S.

AU - Egnuni, T.

AU - Hardegger, R.

AU - Marg-Haufe, B.

AU - Raeber, A.

AU - Oesch, B.

PY - 2010/8/28

Y1 - 2010/8/28

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AB - In this study, interferon-γ (IFN-γ) responses in whole blood cultures stimulated with tuberculins from different sources were compared with regard to their diagnostic reliability in cattle experimentally and naturally infected with Mycobacterium bovis. The IFN-γ responses to different concentrations of purified protein derivatives (PPDs) from M bovis and Mycobacterium avium were quantified. Significant differences (P<0.05) between sources and concentrations of PPDs used for stimulation were detected, indicating a need for standardisation of PPDs used in the IFN-γ assay. Additionally, a tool named 'relative potency 30' that allows rapid comparison of batches and sources of PPDs was defined.

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SN - 0042-4900

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EP - 326

JO - Veterinary Record

JF - Veterinary Record

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Comparison of tuberculin activity using the interferon-γ assay for the diagnosis of bovine tuberculosis

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