Comparison of tuberculin activity using the interferon-γ assay for the diagnosis of bovine tuberculosis

I. Schiller; H. M. Vordermeier; W. R. Waters; A. Kyburz; M. Cagiola; A. Whelan; M. V. Palmer; T. C. Thacker; J. Meijlis; C. Carter; S. Gordon; T. Egnuni; R. Hardegger; B. Marg-Haufe; A. Raeber; B. Oesch

doi:10.1136/vr.c3403

Comparison of tuberculin activity using the interferon-γ assay for the diagnosis of bovine tuberculosis

I. Schiller
, H. M. Vordermeier
, W. R. Waters
, A. Kyburz
, M. Cagiola
, A. Whelan
, M. V. Palmer
, T. C. Thacker
, J. Meijlis
, C. Carter
, S. Gordon
, T. Egnuni
, R. Hardegger
, B. Marg-Haufe
, A. Raeber
, B. Oesch^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

In this study, interferon-γ (IFN-γ) responses in whole blood cultures stimulated with tuberculins from different sources were compared with regard to their diagnostic reliability in cattle experimentally and naturally infected with Mycobacterium bovis. The IFN-γ responses to different concentrations of purified protein derivatives (PPDs) from M bovis and Mycobacterium avium were quantified. Significant differences (P<0.05) between sources and concentrations of PPDs used for stimulation were detected, indicating a need for standardisation of PPDs used in the IFN-γ assay. Additionally, a tool named 'relative potency 30' that allows rapid comparison of batches and sources of PPDs was defined.

Original language	English
Pages (from-to)	322-326
Number of pages	5
Journal	Veterinary Record
Volume	167
Issue number	9
DOIs	https://doi.org/10.1136/vr.c3403
Publication status	Published - 28 Aug 2010

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Schiller, I., Vordermeier, H. M., Waters, W. R., Kyburz, A., Cagiola, M., Whelan, A., Palmer, M. V., Thacker, T. C., Meijlis, J., Carter, C., Gordon, S., Egnuni, T., Hardegger, R., Marg-Haufe, B., Raeber, A., & Oesch, B. (2010). Comparison of tuberculin activity using the interferon-γ assay for the diagnosis of bovine tuberculosis. Veterinary Record, 167(9), 322-326. https://doi.org/10.1136/vr.c3403

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abstract = "In this study, interferon-γ (IFN-γ) responses in whole blood cultures stimulated with tuberculins from different sources were compared with regard to their diagnostic reliability in cattle experimentally and naturally infected with Mycobacterium bovis. The IFN-γ responses to different concentrations of purified protein derivatives (PPDs) from M bovis and Mycobacterium avium were quantified. Significant differences (P<0.05) between sources and concentrations of PPDs used for stimulation were detected, indicating a need for standardisation of PPDs used in the IFN-γ assay. Additionally, a tool named 'relative potency 30' that allows rapid comparison of batches and sources of PPDs was defined.",

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Schiller, I, Vordermeier, HM, Waters, WR, Kyburz, A, Cagiola, M, Whelan, A, Palmer, MV, Thacker, TC, Meijlis, J, Carter, C, Gordon, S, Egnuni, T, Hardegger, R, Marg-Haufe, B, Raeber, A & Oesch, B 2010, 'Comparison of tuberculin activity using the interferon-γ assay for the diagnosis of bovine tuberculosis', Veterinary Record, vol. 167, no. 9, pp. 322-326. https://doi.org/10.1136/vr.c3403

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T1 - Comparison of tuberculin activity using the interferon-γ assay for the diagnosis of bovine tuberculosis

AU - Schiller, I.

AU - Vordermeier, H. M.

AU - Waters, W. R.

AU - Kyburz, A.

AU - Cagiola, M.

AU - Whelan, A.

AU - Palmer, M. V.

AU - Thacker, T. C.

AU - Meijlis, J.

AU - Carter, C.

AU - Gordon, S.

AU - Egnuni, T.

AU - Hardegger, R.

AU - Marg-Haufe, B.

AU - Raeber, A.

AU - Oesch, B.

PY - 2010/8/28

Y1 - 2010/8/28

N2 - In this study, interferon-γ (IFN-γ) responses in whole blood cultures stimulated with tuberculins from different sources were compared with regard to their diagnostic reliability in cattle experimentally and naturally infected with Mycobacterium bovis. The IFN-γ responses to different concentrations of purified protein derivatives (PPDs) from M bovis and Mycobacterium avium were quantified. Significant differences (P<0.05) between sources and concentrations of PPDs used for stimulation were detected, indicating a need for standardisation of PPDs used in the IFN-γ assay. Additionally, a tool named 'relative potency 30' that allows rapid comparison of batches and sources of PPDs was defined.

AB - In this study, interferon-γ (IFN-γ) responses in whole blood cultures stimulated with tuberculins from different sources were compared with regard to their diagnostic reliability in cattle experimentally and naturally infected with Mycobacterium bovis. The IFN-γ responses to different concentrations of purified protein derivatives (PPDs) from M bovis and Mycobacterium avium were quantified. Significant differences (P<0.05) between sources and concentrations of PPDs used for stimulation were detected, indicating a need for standardisation of PPDs used in the IFN-γ assay. Additionally, a tool named 'relative potency 30' that allows rapid comparison of batches and sources of PPDs was defined.

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U2 - 10.1136/vr.c3403

DO - 10.1136/vr.c3403

M3 - Article

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AN - SCOPUS:78049448061

SN - 0042-4900

VL - 167

SP - 322

EP - 326

JO - Veterinary Record

JF - Veterinary Record

IS - 9

ER -

Comparison of tuberculin activity using the interferon-γ assay for the diagnosis of bovine tuberculosis

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