Copia-SSR: A simple marker technique which can be used on total genomic DNA

J. Provan, W. T. B. Thomas, B. P. Forster, W. Powell

Research output: Contribution to journalArticlepeer-review

27 Citations (SciVal)

Abstract

We have developed a PCR-based assay which uses a combination of two classes of repetitive elements found in eukaryotic genomes, namely Ty1-Copia retrotransposons and simple sequence repeats, to amplify multiple polymorphic products from total genomic DNA. Using the technique, seven markers were mapped to four different chromosome arms using a barley doubled haploid mapping population. This technique offers a simple and efficient method of generating both dominant and codominant genetic markers in virtually any eukaryote for mapping and diversity studies.

Original languageEnglish
Pages (from-to)363-366
Number of pages4
JournalGenome
Volume42
Issue number2
DOIs
Publication statusPublished - Apr 1999

Keywords

  • Copia-SSR
  • microsatellite
  • retrotransposon
  • barley
  • genotyping
  • TY1-COPIA GROUP
  • PCR
  • RETROTRANSPOSONS
  • AMPLIFICATION
  • POLYMERASE
  • PLANTS
  • REPEATS
  • Barley
  • Microsatellite
  • Retrotransposon
  • Genotyping

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