Copia-SSR: A simple marker technique which can be used on total genomic DNA

We have developed a PCR-based assay which uses a combination of two classes of repetitive elements found in eukaryotic genomes, namely Ty1-Copia retrotransposons and simple sequence repeats, to amplify multiple polymorphic products from total genomic DNA. Using the technique, seven markers were mapped to four different chromosome arms using a barley doubled haploid mapping population. This technique offers a simple and efficient method of generating both dominant and codominant genetic markers in virtually any eukaryote for mapping and diversity studies.