Cross-species identification of Mendel's/locus

Ian P. Armstead; Iain S. Donnison; Sylvain Aubry; John A. Harper; Stefan Hörtensteiner; Caron L. James; Jan Mani; Matt Moffet; Helen Joan Ougham; Luned Ann Roberts; Ann Myfanwy Thomas; Norman  Weeden; Sid Thomas; Ian Philip King

doi:10.1126/science.1132912

Cross-species identification of Mendel's/locus

Ian P. Armstead, Iain S. Donnison, Sylvain Aubry, John A. Harper, Stefan Hörtensteiner, Caron L. James, Jan Mani, Matt Moffet, Helen Joan Ougham, Luned Ann Roberts, Ann Myfanwy Thomas, Norman Weeden, Sid Thomas, Ian Philip King

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

165 Citations (Scopus)

Abstract

A key gene involved in plant senescence, mutations of which partially disable chlorophyll catabolism and confer stay-green leaf and cotyledon phenotypes, has been identified in Pisum sativum, Arabidopsis thaliana, and Festuca pratensis by using classical and molecular genetics and comparative genomics. A stay-green locus in F. pratensis is syntenically equivalent to a similar stay-green locus on rice chromosome 9. Functional testing in Arabidopsis of a homolog of the rice candidate gene revealed (i) senescence-associated gene expression and (ii) a stay-green phenotype after RNA interference silencing. Genetic mapping in pea demonstrated cosegregation with the yellow/green cotyledon polymorphism (I/i) first reported by Gregor Mendel in 1866.

Original language	English
Pages (from-to)	73
Journal	Science
Volume	315
Issue number	5808
DOIs	https://doi.org/10.1126/science.1132912
Publication status	Published - 05 Jan 2007

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title = "Cross-species identification of Mendel's/locus",

abstract = "A key gene involved in plant senescence, mutations of which partially disable chlorophyll catabolism and confer stay-green leaf and cotyledon phenotypes, has been identified in Pisum sativum, Arabidopsis thaliana, and Festuca pratensis by using classical and molecular genetics and comparative genomics. A stay-green locus in F. pratensis is syntenically equivalent to a similar stay-green locus on rice chromosome 9. Functional testing in Arabidopsis of a homolog of the rice candidate gene revealed (i) senescence-associated gene expression and (ii) a stay-green phenotype after RNA interference silencing. Genetic mapping in pea demonstrated cosegregation with the yellow/green cotyledon polymorphism (I/i) first reported by Gregor Mendel in 1866.",

author = "Armstead, {Ian P.} and Donnison, {Iain S.} and Sylvain Aubry and Harper, {John A.} and Stefan H{\"o}rtensteiner and James, {Caron L.} and Jan Mani and Matt Moffet and Ougham, {Helen Joan} and Roberts, {Luned Ann} and Thomas, {Ann Myfanwy} and Norman Weeden and Sid Thomas and King, {Ian Philip}",

note = "Armstead, I. P., Donnison, I. S., Aubry, S., Harper, J. A., H{\"o}rtensteiner, S., James, C. L., Mani, J., Moffet, M., Ougham, H. J., Roberts, L. A., Thomas, A. M., Weeden, N., Thomas, S., King, I. P. (2007). Cross-species identification of Mendel's/locus. Science, 315 (5808), 73. Sponsorship: BBSRC RAE2008",

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doi = "10.1126/science.1132912",

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T1 - Cross-species identification of Mendel's/locus

AU - Armstead, Ian P.

AU - Donnison, Iain S.

AU - Aubry, Sylvain

AU - Harper, John A.

AU - Hörtensteiner, Stefan

AU - James, Caron L.

AU - Mani, Jan

AU - Moffet, Matt

AU - Ougham, Helen Joan

AU - Roberts, Luned Ann

AU - Thomas, Ann Myfanwy

AU - Weeden, Norman

AU - Thomas, Sid

AU - King, Ian Philip

N1 - Armstead, I. P., Donnison, I. S., Aubry, S., Harper, J. A., Hörtensteiner, S., James, C. L., Mani, J., Moffet, M., Ougham, H. J., Roberts, L. A., Thomas, A. M., Weeden, N., Thomas, S., King, I. P. (2007). Cross-species identification of Mendel's/locus. Science, 315 (5808), 73. Sponsorship: BBSRC RAE2008

PY - 2007/1/5

Y1 - 2007/1/5

N2 - A key gene involved in plant senescence, mutations of which partially disable chlorophyll catabolism and confer stay-green leaf and cotyledon phenotypes, has been identified in Pisum sativum, Arabidopsis thaliana, and Festuca pratensis by using classical and molecular genetics and comparative genomics. A stay-green locus in F. pratensis is syntenically equivalent to a similar stay-green locus on rice chromosome 9. Functional testing in Arabidopsis of a homolog of the rice candidate gene revealed (i) senescence-associated gene expression and (ii) a stay-green phenotype after RNA interference silencing. Genetic mapping in pea demonstrated cosegregation with the yellow/green cotyledon polymorphism (I/i) first reported by Gregor Mendel in 1866.

AB - A key gene involved in plant senescence, mutations of which partially disable chlorophyll catabolism and confer stay-green leaf and cotyledon phenotypes, has been identified in Pisum sativum, Arabidopsis thaliana, and Festuca pratensis by using classical and molecular genetics and comparative genomics. A stay-green locus in F. pratensis is syntenically equivalent to a similar stay-green locus on rice chromosome 9. Functional testing in Arabidopsis of a homolog of the rice candidate gene revealed (i) senescence-associated gene expression and (ii) a stay-green phenotype after RNA interference silencing. Genetic mapping in pea demonstrated cosegregation with the yellow/green cotyledon polymorphism (I/i) first reported by Gregor Mendel in 1866.

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DO - 10.1126/science.1132912

M3 - Article

SN - 1095-9203

VL - 315

SP - 73

JO - Science

JF - Science

IS - 5808

ER -

Cross-species identification of Mendel's/locus

Abstract

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