Crystallization and preliminary crystallographic analysis of a new class of glutathione transferase from nematodes

Q. Hao, D. J. Schuller, John Barrett, Peter M. Brophy, Alison Mary Campbell, I. A. Kriksunov

Research output: Contribution to journalArticlepeer-review

2 Citations (SciVal)

Abstract

Mouse and Heligmosomoides polygyrus constitute a readily manipulated small-animal laboratory model for investigating host-nematode interactions. Two major forms of glutathione transferase (GST) are expressed in H. polygyrus adult worms following primary infection. One of these forms belongs to a new class of GST which has only been found in the nematode phylum and therefore presents a possible target for nematode control. In this study, crystals were obtained of a recombinant representative of this new GST class from H. polygyrus. These crystals belong to the triclinic space group P1, with unit-cell parameters a = 72.7, b = 74.0, c = 88.6 Å, = 79.1, = 80.1, = 81.5°, and are likely to contain four homodimers in the asymmetric unit. X-ray diffraction data were collected to 1.8 Å resolution on station A1 at the Cornell High-Energy Synchrotron Source (CHESS).
Original languageEnglish
Pages (from-to)1262-1264
Number of pages3
JournalActa Crystallographica Section D: Biological Crystallography
Volume59
Issue number7
DOIs
Publication statusPublished - 2003

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