Cupid, a cell permeable peptide derived from amoeba, capable of delivering GFP into a diverse range of species

Daniel Fenton, Dylan Phillips, Anne Maddison, Christopher H. George, Jonathan Ryves, Huw D. Jones*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Citations (SciVal)
132 Downloads (Pure)

Abstract

Cell permeating peptides (CPPs) are attracting great interest for use as molecular delivery vehicles for the transport of biologically active cargo across the cell membrane. The sequence of a novel CPP sequence, termed ‘Cupid’, was identified from the genome of Dictyostelium discoideum. A Cupid-Green Fluorescent Protein (Cupid-GFP) fusion protein was tested on mammalian, whole plant cells, plant leaf protoplast and fungal cell cultures and observed using confocal microscopy. GFP fluorescence builds up within the cell cytosol in 60 min, demonstrating Cupid-GFP has permeated them and folded correctly into its fluorescent form. Our combined data suggest Cupid can act as a molecular vehicle capable of delivering proteins, such as GFP, into the cytosol of a variety of cells.

Original languageEnglish
Article number13725
Number of pages11
JournalScientific Reports
Volume10
Issue number1
DOIs
Publication statusPublished - 13 Aug 2020

Keywords

  • Amoeba/metabolism
  • Animals
  • Cell Membrane/metabolism
  • Cell-Penetrating Peptides/metabolism
  • Cells, Cultured
  • Cytosol/metabolism
  • Dictyostelium/metabolism
  • Fluorescence
  • Green Fluorescent Proteins/metabolism
  • Mice

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