Projects per year
Cell permeating peptides (CPPs) are attracting great interest for use as molecular delivery vehicles for the transport of biologically active cargo across the cell membrane. The sequence of a novel CPP sequence, termed ‘Cupid’, was identified from the genome of Dictyostelium discoideum. A Cupid-Green Fluorescent Protein (Cupid-GFP) fusion protein was tested on mammalian, whole plant cells, plant leaf protoplast and fungal cell cultures and observed using confocal microscopy. GFP fluorescence builds up within the cell cytosol in 60 min, demonstrating Cupid-GFP has permeated them and folded correctly into its fluorescent form. Our combined data suggest Cupid can act as a molecular vehicle capable of delivering proteins, such as GFP, into the cytosol of a variety of cells.
|Number of pages||11|
|Publication status||Published - 13 Aug 2020|
- Cell Membrane/metabolism
- Cell-Penetrating Peptides/metabolism
- Cells, Cultured
- Green Fluorescent Proteins/metabolism
FingerprintDive into the research topics of 'Cupid, a cell permeable peptide derived from amoeba, capable of delivering GFP into a diverse range of species'. Together they form a unique fingerprint.
- Faculty of Earth and Life Sciences, Department of Life Sciences - Chair in Translational Genomics for Plant Breeding
Person: Teaching And Research
- 1 Finished
BBSRC Core Strategic Programme in Resilient Crops: Grasslands Gogerddan
Armstead, I., Donnison, I., Jones, H., Skot, L., Fernandez Fuentes, N., Phillips, D., Kingston-Smith, A. & Bosch, M.
Biotechnology and Biological Sciences Research Council
01 Apr 2017 → 31 Mar 2020
Project: Externally funded research