TY - JOUR
T1 - Cyclophilin A is a new M cell marker of bovine intestinal epithelium
AU - Hondo, Tetsuya
AU - Someya, Shunsuke
AU - Nagasawa, Yuya
AU - Terada, Shunsuke
AU - Watanabe, Hitoshi
AU - Chen, Xiangning
AU - Watanabe, Kouichi
AU - Ohwada, Shyuichi
AU - Kitazawa, Haruki
AU - Rose, Michael
AU - Nochi, Tomonori
AU - Aso, Hisashi
N1 - This is the author accepted manuscript. The final version is available from Springer Verlag via http://dx.doi.org/10.1007/s00441-015-2342-1
PY - 2016/6/1
Y1 - 2016/6/1
N2 - Microfold (M) cells in the follicle-associated epithelium (FAE) of Peyer’s patches contribute to the mucosal immune response by the transcytosis of microorganisms. The mechanism by which M cells take up microorganisms, and the functional proteins by which they do this, are not clear. In order to explore one such protein, we developed a 2H5-F3 monoclonal antibody (2H5-F3 mAb) through its binding to bovine M cells, and identified the antibody reactive molecule as cyclophilin A (Cyp-A). The localization patterns of Cyp-A were very similar to the localization pattern of cytokeratin (CK) 18-positive M cells. Cyp-A was identified at the luminal surface of CK18-positive M cells in bovine jejunal and ileal FAE. The membranous localization of Cyp-A in the bovine intestinal cell line (BIE cells) increased as cells differentiated toward M cells, as determined by flow cytometry analysis. Additionally, BIE cells released Cyp-A to the extracellular space and the differentiation of BIE cells to M cells increased the secretion of Cyp-A, as determined by western blotting. Accordingly, Cyp-A may be localized in M cells in the small intestinal epithelium of cattle. The rise of the membranous localization and secretion of Cyp-A by differentiation toward M cells indicates that Cyp-A has an important role in the function of M cells. While Cyp-A of the M cell membrane may contribute to the uptake of viruses with peptidyl-prolyl cis-trans isomerase activity, in the extracellular space Cyp-A may work as a chemokine and contribute to the distribution of immuno-competent cells.
AB - Microfold (M) cells in the follicle-associated epithelium (FAE) of Peyer’s patches contribute to the mucosal immune response by the transcytosis of microorganisms. The mechanism by which M cells take up microorganisms, and the functional proteins by which they do this, are not clear. In order to explore one such protein, we developed a 2H5-F3 monoclonal antibody (2H5-F3 mAb) through its binding to bovine M cells, and identified the antibody reactive molecule as cyclophilin A (Cyp-A). The localization patterns of Cyp-A were very similar to the localization pattern of cytokeratin (CK) 18-positive M cells. Cyp-A was identified at the luminal surface of CK18-positive M cells in bovine jejunal and ileal FAE. The membranous localization of Cyp-A in the bovine intestinal cell line (BIE cells) increased as cells differentiated toward M cells, as determined by flow cytometry analysis. Additionally, BIE cells released Cyp-A to the extracellular space and the differentiation of BIE cells to M cells increased the secretion of Cyp-A, as determined by western blotting. Accordingly, Cyp-A may be localized in M cells in the small intestinal epithelium of cattle. The rise of the membranous localization and secretion of Cyp-A by differentiation toward M cells indicates that Cyp-A has an important role in the function of M cells. While Cyp-A of the M cell membrane may contribute to the uptake of viruses with peptidyl-prolyl cis-trans isomerase activity, in the extracellular space Cyp-A may work as a chemokine and contribute to the distribution of immuno-competent cells.
KW - M cell
KW - follicle associated epithelium
KW - Peyer's patch
KW - bovine intestinal epithelium
KW - Cyclophilin A
UR - http://hdl.handle.net/2160/36535
U2 - 10.1007/s00441-015-2342-1
DO - 10.1007/s00441-015-2342-1
M3 - Article
C2 - 26899250
SN - 0302-766X
VL - 364
SP - 585
EP - 597
JO - Cell and Tissue Research
JF - Cell and Tissue Research
IS - 3
ER -