Cytokine mRNA expression in cattle infected with different dosages of Mycobacterium bovis

Hima Chandana Boddu-Jasmine, Jaydene Witchell, Martin Vordermeier, Arun Wangoo, Madhu Goyal*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Citations (SciVal)

Abstract

Cytokine mRNA expression of pro-inflammatory cytokines, i.e., interferon-gamma (IFN-γ), tumor necrosis factor-alpha (TNF-α) and anti-inflammatory cytokines, i.e., interleukin-4 (IL-4), interleukin-10 (IL-10) was quantified using quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) in cattle infected with different doses (1-1000 colony-forming units (cfu)) of Mycobacterium bovis. RNA was extracted from the Hepes glutamic acid buffer mediated organic solvent protection effect (HOPE) fixed lymph node tissues using Trizol method. The expression levels of all the four cytokines gradually increased in cattle infected with 1 cfu-1000 cfu. Statistical significance (P < 0.05) was observed for the cytokines IFN-γ, IL-4 and IL-10 between the cattle infected with 1 cfu and 1000 cfu. Though there was an increase in the expression levels of TNF-α from cattle infected with 1 cfu-1000 cfu, this difference in expression was not statistically significant (P > 0.05). The increase in the levels of IFN-γ indicates that the host may be responding to control the infection and the increased level of IL-4 and IL-10 which are anti-inflammatory cytokines, suggests that these cytokines are trying to protect the host by reducing the inflammation and also by controlling the levels of TNF-α (the cytokine that may cause tissue damage).

Original languageEnglish
Pages (from-to)610-615
Number of pages6
JournalTuberculosis
Volume88
Issue number6
Early online date31 Jul 2008
DOIs
Publication statusPublished - 01 Nov 2008

Keywords

  • Colony-forming units
  • HOPE fixed tissues
  • Mycobacterium bovis
  • Quantitative reverse transcriptase polymerase chain reaction
  • RNA extraction

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