Design, implementation and interpretation of in vitro batch culture experiments to assess enteric methane mitigation in ruminants - a review

D. R. Yáñez-Ruiz, A. Bannink, J. Dijkstra, E. Kebreab, D. P. Morgavi, P. O'Kiely, C. K. Reynolds, A. Schwarm, Kevin Shingfield, Z. Yu, A. N. Hristov

Research output: Contribution to journalArticlepeer-review

106 Citations (SciVal)
250 Downloads (Pure)

Abstract

In vitro fermentation techniques (IVFT) have been widely used to evaluate the nutritive value of feeds for ruminants and in the last decade to assess the effect of different nutritional strategies on methane production. However, many technical factors may influence the results obtained. The present review has been prepared by the ‘Global Network’ FACCE-JPI international research consortium to provide a critical evaluation of the main factors that need to be considered when designing, conducting and interpreting IVFT experiments investigate nutritional strategies to mitigate methane (CH4) emission from ruminants. Given the increasing and wide-scale use of IVFT techniques, there is a need to critically review reports in the literature and establish what criteria are essential to the establishment and implementation of in vitro techniques. Key aspects considered include: i) donor animal species and number of animal used, ii) diet fed to donor animals, iii) collection and processing of rumen fluid as inoculum, iv) choice of substrate and incubation buffer, v) incubation procedures and CH4 measurements, vi) headspace gas composition and vii) comparability of in vitro and in vivo measurements. Based on an evaluation of experimental evidence, a set of technical recommendations are presented that allowing the harmonization of IVFT laboratory methods employed with IVFT, of and procedures for feed evaluation, assessment of rumen function and CH4 production.
Original languageEnglish
Pages (from-to)1-18
JournalAnimal Feed Science and Technology
Volume216
Early online date17 Mar 2016
DOIs
Publication statusPublished - 01 Jun 2016

Keywords

  • DM, dry matter
  • DMI, dry matter intake
  • IVFT, in vitro fermentation technique
  • NDF, neutral detergent fibre
  • OM, organic matter
  • VFA, volatile fatty acids

Fingerprint

Dive into the research topics of 'Design, implementation and interpretation of in vitro batch culture experiments to assess enteric methane mitigation in ruminants - a review'. Together they form a unique fingerprint.

Cite this