TY - JOUR
T1 - Determination of allantoin in bovine milk by high-performance liquid chromatography
AU - Shingfield, K J
AU - Offer, N W
N1 - Funding Information:
The authors would like to thank Mr. K. McIssac for technical assistance during this work. K.J. Shingfield gratefully acknowledges the Ministry of Agriculture, Fisheries and Food for provision of financial support during this work. SAC receives financial support from the Scottish Office Agriculture and Fisheries Department.
PY - 1998/3/20
Y1 - 1998/3/20
N2 - Determination of allantoin in bovine milk based on high-performance liquid chromatography (HPLC) is described. Following dilution and filtration, milk samples were analysed directly. Separation and quantification of allantoin was achieved using a Spherisorb 5 NH
2 column (250x4.6 mm ID), acetonitrile-water (99:10, v/v) mobile phase at a flow-rate of 2.0 ml min
-1, temperature 20°C and monitoring the effluent at 214 nm. Total analysis time was 10 min. Recovery of allantoin added to milk was 97 (±3.7, n = 30)%. Lowest delectable concentration was 1 μmol l
-1. Within-day and between-day variability were less than 3%. Advantages of improved retention, and separation of allantoin, and less complicated sample preparation exist over current methods.
AB - Determination of allantoin in bovine milk based on high-performance liquid chromatography (HPLC) is described. Following dilution and filtration, milk samples were analysed directly. Separation and quantification of allantoin was achieved using a Spherisorb 5 NH
2 column (250x4.6 mm ID), acetonitrile-water (99:10, v/v) mobile phase at a flow-rate of 2.0 ml min
-1, temperature 20°C and monitoring the effluent at 214 nm. Total analysis time was 10 min. Recovery of allantoin added to milk was 97 (±3.7, n = 30)%. Lowest delectable concentration was 1 μmol l
-1. Within-day and between-day variability were less than 3%. Advantages of improved retention, and separation of allantoin, and less complicated sample preparation exist over current methods.
KW - Allantoin
KW - Animals
KW - Chromatography, High Pressure Liquid
KW - Circadian Rhythm
KW - Milk
KW - Reproducibility of Results
KW - Sensitivity and Specificity
KW - Spectrophotometry, Ultraviolet
UR - http://www.scopus.com/inward/record.url?scp=0032549476&partnerID=8YFLogxK
U2 - 10.1016/S0378-4347(97)00549-5
DO - 10.1016/S0378-4347(97)00549-5
M3 - Article
C2 - 9551822
SN - 1387-2273
VL - 706
SP - 342
EP - 346
JO - Journal of chromatography B: Biomedical Sciences and Applications
JF - Journal of chromatography B: Biomedical Sciences and Applications
IS - 2
ER -