Determination of allantoin in bovine milk by high-performance liquid chromatography

K J Shingfield, N W Offer

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19 Citations (SciVal)


Determination of allantoin in bovine milk based on high-performance liquid chromatography (HPLC) is described. Following dilution and filtration, milk samples were analysed directly. Separation and quantification of allantoin was achieved using a Spherisorb 5 NH 2 column (250x4.6 mm ID), acetonitrile-water (99:10, v/v) mobile phase at a flow-rate of 2.0 ml min -1, temperature 20°C and monitoring the effluent at 214 nm. Total analysis time was 10 min. Recovery of allantoin added to milk was 97 (±3.7, n = 30)%. Lowest delectable concentration was 1 μmol l -1. Within-day and between-day variability were less than 3%. Advantages of improved retention, and separation of allantoin, and less complicated sample preparation exist over current methods.

Original languageEnglish
Pages (from-to)342-346
Number of pages5
JournalJournal of chromatography B: Biomedical Sciences and Applications
Issue number2
Publication statusPublished - 20 Mar 1998


  • Allantoin
  • Animals
  • Chromatography, High Pressure Liquid
  • Circadian Rhythm
  • Milk
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Spectrophotometry, Ultraviolet


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