Developing a food exposure and urine sampling strategy for dietary exposure biomarker validation in free-living individuals

Amanda Lloyd; Naomi D. Willis; Thomas Wilson; Hassan Zubair; Long Xie; Edward S. Chambers; Isabel Garcia-Perez; Kathleen Tailliart; Manfred Beckmann; John C. Mathers; John Draper

doi:10.1002/mnfr.201900062

Developing a food exposure and urine sampling strategy for dietary exposure biomarker validation in free-living individuals

Amanda Lloyd, Naomi D. Willis, Thomas Wilson, Hassan Zubair, Long Xie, Edward S. Chambers, Isabel Garcia-Perez, Kathleen Tailliart, Manfred Beckmann, John C. Mathers, John Draper

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

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Abstract

Scope
Dietary choices modulate the risk of chronic diseases and improving diet is a central component of public health strategies. Food-derived metabolites present in urine could provide objective biomarkers of dietary exposure. To assist biomarker validation, this work aims to develop a food intervention strategy mimicking a typical annual diet over a short period of time and assesses urine sampling protocols potentially suitable for future deployment of biomarker technology in free-living populations.

Methods and results
Six different menu plans comprehensively represent a typical UK annual diet that is split into two dietary experimental periods. Free-living adult participants (n = 15 and n = 36, respectively) are provided with all their food, as a series of menu plans, over a period of three consecutive days. Multiple spot urine samples are collected and stored at home.

Conclusion
A successful food exposure strategy is established following a conventional UK eating pattern, which is suitable for biomarker validation in free-living individuals. The urine sampling procedure is acceptable for volunteers and delivered samples suitable for biomarker quantification. The study design provides scope for validation of existing biomarker candidates and potentially for discovery of new biomarker leads, and should help inform the future deployment of biomarker technology for habitual dietary exposure measurement.

Original language	English
Article number	1900062
Journal	Molecular Nutrition and Food Research
Volume	63
Issue number	14
Early online date	17 Jun 2019
DOIs	https://doi.org/10.1002/mnfr.201900062
Publication status	Published - 19 Jul 2019

Keywords

dietary-exposure
urine-sampling
biomarker
free-living
metabolomics
dietary exposure
biomarkers
urine sampling
Humans
Middle Aged
Male
United Kingdom
Young Adult
Diet
Biomarkers/urine
Adult
Female
Aged
Acidosis
Urine Specimen Collection/methods
Food

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Developing a Food Exposure and Urine Sampling Strategy for Dietary Exposure Biomarker Validation in Free-Living IndividualsFinal published version, 655 KBLicence: CC BY

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Lloyd, A., Willis, N. D., Wilson, T., Zubair, H., Xie, L., Chambers, E. S., Garcia-Perez, I., Tailliart, K., Beckmann, M., Mathers, J. C., & Draper, J. (2019). Developing a food exposure and urine sampling strategy for dietary exposure biomarker validation in free-living individuals. Molecular Nutrition and Food Research, 63(14), Article 1900062. https://doi.org/10.1002/mnfr.201900062

@article{4c76ec5098454f9ba308d7335b7abb37,

title = "Developing a food exposure and urine sampling strategy for dietary exposure biomarker validation in free-living individuals",

abstract = "ScopeDietary choices modulate the risk of chronic diseases and improving diet is a central component of public health strategies. Food-derived metabolites present in urine could provide objective biomarkers of dietary exposure. To assist biomarker validation, this work aims to develop a food intervention strategy mimicking a typical annual diet over a short period of time and assesses urine sampling protocols potentially suitable for future deployment of biomarker technology in free-living populations.Methods and resultsSix different menu plans comprehensively represent a typical UK annual diet that is split into two dietary experimental periods. Free-living adult participants (n = 15 and n = 36, respectively) are provided with all their food, as a series of menu plans, over a period of three consecutive days. Multiple spot urine samples are collected and stored at home.ConclusionA successful food exposure strategy is established following a conventional UK eating pattern, which is suitable for biomarker validation in free-living individuals. The urine sampling procedure is acceptable for volunteers and delivered samples suitable for biomarker quantification. The study design provides scope for validation of existing biomarker candidates and potentially for discovery of new biomarker leads, and should help inform the future deployment of biomarker technology for habitual dietary exposure measurement.",

keywords = "dietary-exposure, urine-sampling, biomarker, free-living, metabolomics, dietary exposure, biomarkers, urine sampling, Humans, Middle Aged, Male, United Kingdom, Young Adult, Diet, Biomarkers/urine, Adult, Female, Aged, Acidosis, Urine Specimen Collection/methods, Food",

author = "Amanda Lloyd and Willis, {Naomi D.} and Thomas Wilson and Hassan Zubair and Long Xie and Chambers, {Edward S.} and Isabel Garcia-Perez and Kathleen Tailliart and Manfred Beckmann and Mathers, {John C.} and John Draper",

note = "Funding Information: A.J.L. and N.D.W. contributed equally to this work. The authors thank the volunteers for their commitment and the CARU in Newcastle for volunteer support. This study was funded by the Medical Research Council (MRC), grant number MR/J010308/1. The authors{\textquoteright} contributions to the work were as follows: A.J.L., data analysis, produced figures, researched literature, and wrote the manuscript; N.D.W., undertook volunteer recruitment, coordinated volunteer CRF visits and supervised CRF support staff, refined sampling methodology, and edited the manuscript; T.W., created and utilized bespoke routines for multivariate modelling, classification, and feature selection and QQQ data; H.Z., QQQ technical support; L.X., provided volunteer handling support and technical support in Newcastle; E.H. and G.F. coordinated project input from Imperial College; K.T., FIE-HRMS analysis and technical support in Aberystwyth; M.B., developed urine extraction procedures, designed metabolite fingerprinting experiments, supervised MS support staff, and edited the manuscript; J.C.M., co-ordinated project, supervised research in Newcastle University, designed volunteer handling protocols, and edited the manuscript and J.D. coordinated project, supervised research in Aberystwyth, designed figures, and wrote the manuscript. Funding Information: A.J.L. and N.D.W. contributed equally to this work. The authors thank the volunteers for their commitment and the CARU in Newcastle for volunteer support. This study was funded by the Medical Research Council (MRC), grant number MR/J010308/1. The authors? contributions to the work were as follows: A.J.L., data analysis, produced figures, researched literature, and wrote the manuscript; N.D.W., undertook volunteer recruitment, coordinated volunteer CRF visits and supervised CRF support staff, refined sampling methodology, and edited the manuscript; T.W., created and utilized bespoke routines for multivariate modelling, classification, and feature selection and QQQ data; H.Z., QQQ technical support; L.X., provided volunteer handling support and technical support in Newcastle; E.H. and G.F. coordinated project input from Imperial College; K.T., FIE-HRMS analysis and technical support in Aberystwyth; M.B., developed urine extraction procedures, designed metabolite fingerprinting experiments, supervised MS support staff, and edited the manuscript; J.C.M., coordinated project, supervised research in Newcastle University, designed volunteer handling protocols, and edited the manuscript and J.D. coordinated project, supervised research in Aberystwyth, designed figures, and wrote the manuscript. Publisher Copyright: {\textcopyright} 2019 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.",

year = "2019",

month = jul,

day = "19",

doi = "10.1002/mnfr.201900062",

language = "English",

volume = "63",

journal = "Molecular Nutrition and Food Research",

issn = "1613-4125",

publisher = "Wiley",

number = "14",

}

Lloyd, A, Willis, ND, Wilson, T, Zubair, H, Xie, L, Chambers, ES, Garcia-Perez, I, Tailliart, K, Beckmann, M, Mathers, JC & Draper, J 2019, 'Developing a food exposure and urine sampling strategy for dietary exposure biomarker validation in free-living individuals', Molecular Nutrition and Food Research, vol. 63, no. 14, 1900062. https://doi.org/10.1002/mnfr.201900062

TY - JOUR

T1 - Developing a food exposure and urine sampling strategy for dietary exposure biomarker validation in free-living individuals

AU - Lloyd, Amanda

AU - Willis, Naomi D.

AU - Wilson, Thomas

AU - Zubair, Hassan

AU - Xie, Long

AU - Chambers, Edward S.

AU - Garcia-Perez, Isabel

AU - Tailliart, Kathleen

AU - Beckmann, Manfred

AU - Mathers, John C.

AU - Draper, John

N1 - Funding Information: A.J.L. and N.D.W. contributed equally to this work. The authors thank the volunteers for their commitment and the CARU in Newcastle for volunteer support. This study was funded by the Medical Research Council (MRC), grant number MR/J010308/1. The authors’ contributions to the work were as follows: A.J.L., data analysis, produced figures, researched literature, and wrote the manuscript; N.D.W., undertook volunteer recruitment, coordinated volunteer CRF visits and supervised CRF support staff, refined sampling methodology, and edited the manuscript; T.W., created and utilized bespoke routines for multivariate modelling, classification, and feature selection and QQQ data; H.Z., QQQ technical support; L.X., provided volunteer handling support and technical support in Newcastle; E.H. and G.F. coordinated project input from Imperial College; K.T., FIE-HRMS analysis and technical support in Aberystwyth; M.B., developed urine extraction procedures, designed metabolite fingerprinting experiments, supervised MS support staff, and edited the manuscript; J.C.M., co-ordinated project, supervised research in Newcastle University, designed volunteer handling protocols, and edited the manuscript and J.D. coordinated project, supervised research in Aberystwyth, designed figures, and wrote the manuscript. Funding Information: A.J.L. and N.D.W. contributed equally to this work. The authors thank the volunteers for their commitment and the CARU in Newcastle for volunteer support. This study was funded by the Medical Research Council (MRC), grant number MR/J010308/1. The authors? contributions to the work were as follows: A.J.L., data analysis, produced figures, researched literature, and wrote the manuscript; N.D.W., undertook volunteer recruitment, coordinated volunteer CRF visits and supervised CRF support staff, refined sampling methodology, and edited the manuscript; T.W., created and utilized bespoke routines for multivariate modelling, classification, and feature selection and QQQ data; H.Z., QQQ technical support; L.X., provided volunteer handling support and technical support in Newcastle; E.H. and G.F. coordinated project input from Imperial College; K.T., FIE-HRMS analysis and technical support in Aberystwyth; M.B., developed urine extraction procedures, designed metabolite fingerprinting experiments, supervised MS support staff, and edited the manuscript; J.C.M., coordinated project, supervised research in Newcastle University, designed volunteer handling protocols, and edited the manuscript and J.D. coordinated project, supervised research in Aberystwyth, designed figures, and wrote the manuscript. Publisher Copyright: © 2019 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

PY - 2019/7/19

Y1 - 2019/7/19

N2 - ScopeDietary choices modulate the risk of chronic diseases and improving diet is a central component of public health strategies. Food-derived metabolites present in urine could provide objective biomarkers of dietary exposure. To assist biomarker validation, this work aims to develop a food intervention strategy mimicking a typical annual diet over a short period of time and assesses urine sampling protocols potentially suitable for future deployment of biomarker technology in free-living populations.Methods and resultsSix different menu plans comprehensively represent a typical UK annual diet that is split into two dietary experimental periods. Free-living adult participants (n = 15 and n = 36, respectively) are provided with all their food, as a series of menu plans, over a period of three consecutive days. Multiple spot urine samples are collected and stored at home.ConclusionA successful food exposure strategy is established following a conventional UK eating pattern, which is suitable for biomarker validation in free-living individuals. The urine sampling procedure is acceptable for volunteers and delivered samples suitable for biomarker quantification. The study design provides scope for validation of existing biomarker candidates and potentially for discovery of new biomarker leads, and should help inform the future deployment of biomarker technology for habitual dietary exposure measurement.

AB - ScopeDietary choices modulate the risk of chronic diseases and improving diet is a central component of public health strategies. Food-derived metabolites present in urine could provide objective biomarkers of dietary exposure. To assist biomarker validation, this work aims to develop a food intervention strategy mimicking a typical annual diet over a short period of time and assesses urine sampling protocols potentially suitable for future deployment of biomarker technology in free-living populations.Methods and resultsSix different menu plans comprehensively represent a typical UK annual diet that is split into two dietary experimental periods. Free-living adult participants (n = 15 and n = 36, respectively) are provided with all their food, as a series of menu plans, over a period of three consecutive days. Multiple spot urine samples are collected and stored at home.ConclusionA successful food exposure strategy is established following a conventional UK eating pattern, which is suitable for biomarker validation in free-living individuals. The urine sampling procedure is acceptable for volunteers and delivered samples suitable for biomarker quantification. The study design provides scope for validation of existing biomarker candidates and potentially for discovery of new biomarker leads, and should help inform the future deployment of biomarker technology for habitual dietary exposure measurement.

KW - dietary-exposure

KW - urine-sampling

KW - biomarker

KW - free-living

KW - metabolomics

KW - dietary exposure

KW - biomarkers

KW - urine sampling

KW - Humans

KW - Middle Aged

KW - Male

KW - United Kingdom

KW - Young Adult

KW - Diet

KW - Biomarkers/urine

KW - Adult

KW - Female

KW - Aged

KW - Acidosis

KW - Urine Specimen Collection/methods

KW - Food

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U2 - 10.1002/mnfr.201900062

DO - 10.1002/mnfr.201900062

M3 - Article

C2 - 31157514

SN - 1613-4125

VL - 63

JO - Molecular Nutrition and Food Research

JF - Molecular Nutrition and Food Research

IS - 14

M1 - 1900062

ER -

Developing a food exposure and urine sampling strategy for dietary exposure biomarker validation in free-living individuals

Abstract

Keywords

UN SDGs

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John Draper

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Developing a food exposure and urine sampling strategy for dietary exposure biomarker validation in free-living individuals

Abstract

Keywords

UN SDGs

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John Draper

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