Development of a Mycobacterium bovis intranasal challenge model in mice

Karen E. Logan, Dolores Gavier-Widen, R. Glyn Hewinson, Philip J. Hogarth*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

26 Citations (SciVal)

Abstract

Tuberculosis caused by infection with Mycobacterium tuberculosis or Mycobacterium bovis remains one of the most important infectious diseases of man and animals. The current vaccine, M. bovis bacille Calmette-Guérin (BCG) demonstrates variable efficacy in humans and cattle, and so an urgent need exists for a new vaccine to replace or supplement BCG. Novel vaccine development requires the availability of a suitable animal model in which to test potential vaccine candidates. Models for tuberculosis vaccine development include mice, guinea pigs, cattle and non-human primates. Murine models provide an economical and easily manipulated tool, but the natural aerosol infection route requires extensive facilities, equipment and validation. We sought to develop a logistically simpler intranasal M. bovis infection model for use in vaccine development for bovine tuberculosis. Intranasal M. bovis infection model in mice demonstrated distinct airway associated, dose related pathology, and was strikingly more virulent than previously employed intravenous infection with M. bovis. BCG vaccination of intranasal challenged mice induced 2 logs of protection with similar kinetics as those displayed in M. tuberculosis aerosol infection models. In conclusion, we report the development of a virulent, robust, stringent, physiological and inexpensive M. bovis intranasal infection model for the screening of potential vaccine candidates against bovine tuberculosis. Crown

Original languageEnglish
Pages (from-to)437-443
Number of pages7
JournalTuberculosis
Volume88
Issue number5
Early online date30 Jun 2008
DOIs
Publication statusPublished - 01 Sept 2008

Keywords

  • Intranasal
  • Model
  • Mouse
  • Mycobacterium bovis
  • Vaccine

Fingerprint

Dive into the research topics of 'Development of a Mycobacterium bovis intranasal challenge model in mice'. Together they form a unique fingerprint.

Cite this