Development of a simple, sensitive, rapid test which discriminates BCG-vaccinated from Mycobacterium bovis-infected cattle

P. Sopp, M. Coad, G. Hewinson, C. J. Howard, C. Ni Cheallaigh, J. Keane, J. Harris, J. C. Hope*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Citations (SciVal)

Abstract

Bovine tuberculosis (bTB) is increasing in incidence in the UK. Effective control strategies could involve vaccination; BCG, either alone or in prime-boost strategies, remains the most effective vaccine against bovine tuberculosis. However, BCG vaccination of cattle would require development of diagnostic tests able to accurately discriminate Mycobacterium bovis-infected from BCG-vaccinated animals. Herein, we demonstrate that the detection of secreted IFN-γ following short term culture (4 h) of whole blood with purified protein derived from M. bovis (PPD-B) allows such discrimination. This reflects, in part, the differential kinetics of IFN-γ secretion in infected compared to vaccinated cattle. This is the first study to demonstrate that accurate, rapid distinction of BCG-vaccinated from M. bovis-infected cattle can be achieved in a short time period without the need for production of M. bovis-specific antigens, complex antigen mixtures or extensive laboratory procedures. We were also able to detect PPD-specific IFN-γ release during short term culture of blood from a number of humans with active TB indicating that this test may have wider application and is potentially useful for the rapid diagnosis of disease in humans.

Original languageEnglish
Pages (from-to)5470-5476
Number of pages7
JournalVaccine
Volume26
Issue number43
Early online date08 Aug 2008
DOIs
Publication statusPublished - 09 Oct 2008

Keywords

  • Diagnosis
  • Interferon gamma
  • Mycobacterium bovis

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