TY - JOUR
T1 - Different strains of Saccharomyces cerevisiae differ in their effects on ruminal bacterial numbers in vitro and in sheep
AU - Newbold, Jamie
AU - Wallace, R. J.
AU - Chen, X. B.
AU - McIntosh, F. M.
N1 - Newbold, J., Wallace, R. J., Chen, X. B., McIntosh, F. M. (1995). Different strains of Saccharomyces cerevisiae differ in their effects on ruminal bacterial numbers in vitro and in sheep. Journal of Animal Science, 73 (6), 1811-1818.
PY - 1995/6/1
Y1 - 1995/6/1
N2 - A ruminal simulation device (Rusitec) was used to compare the effects of Saccharomyces cerevisiae strains NCYC 240, NCYC 694, NCYC 1026, NCYC 1088, and Yea-Sacc (a commercial product containing S. cerevisiae) on ruminal fermentation. S. cerevisiae NCYC 240, NCYC 1088, NCYC 1026, and NCYC 694 were grown on malt extract at 30 degrees C in aerated fed-batch culture and harvested along with spent growth medium by freeze-drying. Each vessel received daily 20 g of a basal diet consisting of hay, barley, molasses, fishmeal, and a minerals/vitamins mixture at 500, 299.5, 100, 91, and 9.5 g/kg of DM, respectively. Yeast preparations (500 mg/d) were added along with the feed. S. cerevisiae NCYC 240, NCYC 1026, and Yea-Sacc stimulated total and cellulolytic bacterial numbers, whereas S. cerevisiae NCYC 694 and NCYC 1088 had no effect on the numbers of bacteria. The effects of S. cerevisiae NCYC 240, NCYC 1026, and Yea-Sacc on ruminal fermentation were further investigated in vivo using ruminally cannulated sheep fed 1.5 kg/d of the diet used in Rusitec, supplemented with 2 g/d of yeast culture. All treatments tended to stimulate total and cellulolytic bacterial numbers. However, the stimulation was only statistically significant for S. cerevisiae NCYC 1026 with total bacterial numbers and S. cervisiae NCYC 240 with cellulolytic bacteria (P <.05). Increased bacterial numbers were associated with an increase in the rate of straw degradation in the rumen and a nonsignificant(P > .05) increase in the excretion of purine derivatives in the urine, measured as an index of microbial nitrogen leaving the rumen. Thus, differences exist between strains of S. cerevisiae in their ability to modify the rumen bacterial population.
AB - A ruminal simulation device (Rusitec) was used to compare the effects of Saccharomyces cerevisiae strains NCYC 240, NCYC 694, NCYC 1026, NCYC 1088, and Yea-Sacc (a commercial product containing S. cerevisiae) on ruminal fermentation. S. cerevisiae NCYC 240, NCYC 1088, NCYC 1026, and NCYC 694 were grown on malt extract at 30 degrees C in aerated fed-batch culture and harvested along with spent growth medium by freeze-drying. Each vessel received daily 20 g of a basal diet consisting of hay, barley, molasses, fishmeal, and a minerals/vitamins mixture at 500, 299.5, 100, 91, and 9.5 g/kg of DM, respectively. Yeast preparations (500 mg/d) were added along with the feed. S. cerevisiae NCYC 240, NCYC 1026, and Yea-Sacc stimulated total and cellulolytic bacterial numbers, whereas S. cerevisiae NCYC 694 and NCYC 1088 had no effect on the numbers of bacteria. The effects of S. cerevisiae NCYC 240, NCYC 1026, and Yea-Sacc on ruminal fermentation were further investigated in vivo using ruminally cannulated sheep fed 1.5 kg/d of the diet used in Rusitec, supplemented with 2 g/d of yeast culture. All treatments tended to stimulate total and cellulolytic bacterial numbers. However, the stimulation was only statistically significant for S. cerevisiae NCYC 1026 with total bacterial numbers and S. cervisiae NCYC 240 with cellulolytic bacteria (P <.05). Increased bacterial numbers were associated with an increase in the rate of straw degradation in the rumen and a nonsignificant(P > .05) increase in the excretion of purine derivatives in the urine, measured as an index of microbial nitrogen leaving the rumen. Thus, differences exist between strains of S. cerevisiae in their ability to modify the rumen bacterial population.
KW - ACID
KW - SIMULATION-TECHNIQUE RUSITEC
KW - PROBIOTICS
KW - ORYZAE FERMENTATION EXTRACT
KW - YEASTS
KW - SACCHAROMYCES CEREVISIAE
KW - MONENSIN
KW - PURINE DERIVATIVES
KW - YEAST CULTURE SUPPLEMENT
KW - GROWTH
KW - DIETARY INCLUSION
KW - RUMINANTS
KW - DAIRY-COWS
KW - RUMEN FERMENTATION
UR - http://www.scopus.com/inward/record.url?scp=0029314823&partnerID=8YFLogxK
U2 - 10.2527/1995.7361811x
DO - 10.2527/1995.7361811x
M3 - Article
SN - 0021-8812
VL - 73
SP - 1811
EP - 1818
JO - Journal of Animal Science
JF - Journal of Animal Science
IS - 6
ER -