Differential growth of the fungus Absidia cylindrospora on C-13/N-15-labelled media

F. V. Crotty*, R. P. Blackshaw, Philip J. Murray

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Citations (SciVal)

Abstract

Many studies utilise enrichment of stable isotopes as tracers to follow the interactions occurring within soil food webs and methods have been developed to enrich bacteria, soil fauna and plant litter, Here for the first time we attempt to enrich a soil fungus to 99 atom% with C-13 and N-15 stable isotopes. In this study our objectives were to (a) assess whether the saprotrophic zygomycete fungus Absidia cylindrospora could grow on a medium enriched to 99 atom% with C-13-glucose and N-15-ammonium chloride, (b) to determine the level of enrichment obtained, and (c) to examine the change in growth rate of this fungus while it was growing on the dually enriched medium. To achieve this, the fungus was grown on agar enriched with C-13 and N-15 to 99 atom% and its growth rate monitored. The results showed that A. cylindrospora would grow on the highly labelled growth medium, but that its rate of growth was affected compared with the rate on either natural abundance media or media highly enriched with a single isotope (C-13 or N-15). The implications of these results is that although the fungus is able to utilise these heavier isotopes, the biochemical processes involved in growth are affected, and consideration should be given to these differences when using stable isotope tracers in, for example, soil food web studies. Copyright (C) 2011 John Wiley & Sons, Ltd.

Original languageEnglish
Pages (from-to)1479-1484
Number of pages6
JournalRapid Communications in Mass Spectrometry
Volume25
Issue number11
Early online date11 May 2011
DOIs
Publication statusPublished - 15 Jun 2011
EventCombined Meetings of the BMSS Special Interest Group on Stable Isotopes (BMSS ISO-SIG)/ Stable Isotopes Mass Spectrometry Users Group (SIMSUG)/ Stable Isotopes in Biosphere-Atmosphere-Earth System Research (SIBAE) - Exeter
Duration: 27 Apr 201030 Apr 2010

Keywords

  • ECTOMYCORRHIZAL FUNGI
  • CARBON
  • NATURAL-ABUNDANCE
  • SOIL FOOD-WEB
  • FIELD-COLLECTED FUNGI
  • NITROGEN
  • MYCORRHIZAL FUNGI
  • STABLE-ISOTOPE FRACTIONATION
  • C-13
  • N-15 ABUNDANCE

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