Differential responses of epithelial cells from urinary and biliary tract to eggs of Schistosoma haematobium and S. mansoni

Rafael Nacif-Pimenta, Alessandra da Silva Orfanó, Ilana A. Mosley, Shannon E. Karinshak, Kenji Ishida, Victoria H. Mann, Paulo Marcos Zech Coelho, José M.Correia da Costa, Michael H. Hsieh, Paul J. Brindley*, Gabriel Rinaldi

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Citations (SciVal)

Abstract

Chronic urogenital schistosomiasis can lead to squamous cell carcinoma of the bladder. The International Agency for Research on Cancer classifies the infection with S. haematobium as a group 1 carcinogen, a definitive cause of cancer. By contrast, hepatointestinal schistosomiasis due to the chronic infection with S. mansoni or S. japonicum associated with liver periportal fibrosis, does not apparently lead to malignancy. The effects of culturing human epithelial cells, HCV29, established from normal urothelium, and H69, established from cholangiocytes, in the presence of S. haematobium or S. mansoni eggs were investigated. Cell growth of cells co-cultured with schistosome eggs was monitored in real time, and gene expression analysis of oncogenesis, epithelial to mesenchymal transition and apoptosis pathways was undertaken. Schistosome eggs promoted proliferation of the urothelial cells but inhibited growth of cholangiocytes. In addition, the tumor suppressor P53 pathway was significantly downregulated when exposed to schistosome eggs, and downregulation of estrogen receptor was predicted in urothelial cells exposed only to S. haematobium eggs. Overall, cell proliferative responses were influenced by both the tissue origin of the epithelial cells and the schistosome species.

Original languageEnglish
Article number10731
JournalScientific Reports
Volume9
Issue number1
DOIs
Publication statusPublished - 24 Jul 2019
Externally publishedYes

Keywords

  • Animals
  • Biliary Tract/metabolism
  • Cell Line
  • Coculture Techniques
  • Colorectal Neoplasms/metabolism
  • Epithelium/metabolism
  • Estradiol/metabolism
  • Humans
  • Ovum
  • Receptors, Estrogen/metabolism
  • Schistosoma haematobium
  • Schistosoma mansoni
  • Schistosomiasis haematobia/pathology
  • Schistosomiasis mansoni/pathology
  • Signal Transduction
  • Transcriptome
  • Tumor Suppressor Protein p53/metabolism
  • Urothelium/metabolism

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