Differentiation of a murine intestinal epithelial cell line (MIE) towards the M cell lineage

Takashi Kanaya, Kohtaro Miyazawa, Ikuro Takakura, Wataru Itani, Kouichi Watanabe, Shyuichi Ohwada, Haruki Kitazawa, M. T. Rose, H. R. McConochie, Hideyuki Okano, Takahiro Yamaguchi, Hisashi Aso

Research output: Contribution to journalArticlepeer-review

15 Citations (SciVal)


M cells are a kind of intestinal epithelial cell in the follicle-associated epithelium of Peyer's patches. These cells can transport antigens and microorganisms into underlying lymphoid tissues. Despite the important role of M cells in mucosal immune responses, the origin and mechanisms of differentiation as well as cell death of M cells remain unclear. To clarify the mechanism of M cell differentiation, we established a novel murine intestinal epithelial cell line (MIE) from the C57BL/6 mouse. MIE cells grow rapidly and have a cobblestone morphology, which is a typical feature of intestinal epithelial cells. Additionally, they express cytokeratin, villin, cell-cell junctional proteins, and alkaline phosphatase activity and can form microvilli. Their expression of Musashi-1 antigen indicates that they may be close to intestinal stem cells or transit-amplifying cells. MIE cells are able to differentiate into the M cell lineage following coculture with intestinal lymphocytes, but not with Peyer's patch lymphocytes (PPL). However, PPL costimulated with anti-CD3/CD28 MAbs caused MIE cells to display typical features of M cells, such as transcytosis activity, the disorganization of microvilli, and the expression of M cell markers. This transcytosis activity of MIE cells was not induced by T cells isolated from PPL costimulated with the same MAbs and was reduced by the depletion of the T cell population from PPL. A mixture of T cells treated with MAbs and B cells both from PPL led MIE cells to differentiate into M cells. We report here that MIE cells have the potential ability to differentiate into M cells and that this differentiation required activated T cells and B cells.
Original languageEnglish
Pages (from-to)G273-G284
Number of pages12
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Issue number2
Publication statusPublished - 01 Aug 2008


  • M cell
  • MIE cell
  • follicle-associated epithelium
  • Peyer's patch
  • small intestine


Dive into the research topics of 'Differentiation of a murine intestinal epithelial cell line (MIE) towards the M cell lineage'. Together they form a unique fingerprint.

Cite this