TY - JOUR
T1 - Differentiation of Phytophthora infestans sporangia from other airborne biological particles by flow cytometry
AU - Day, Jennifer Penelope
AU - Griffith, Gareth W.
AU - Kell, Douglas B.
N1 - Jennifer P. Day, Douglas B. Kell, and Gareth W. Griffith (2002). Differentiation of Phytophthora infestans sporangia from other airborne biological particles by flow cytometry. Applied and Environmental Microbiology, 68(1), 37-45.
Sponsorship: MAFF
RAE2008
PY - 2002/1/5
Y1 - 2002/1/5
N2 - The ability of two different flow cytometers, the Microcyte (Optoflow) and the PAS-III (Partec), to differentiate sporangia of the late-blight pathogen Phytophthora infestans from other potential airborne particles was compared. With the PAS-III, light scatter and intrinsic fluorescence parameters could be used to differentiate sporangia from conidia of Alternaria or Botrytis spp., rust urediniospores, and pollen of grasses and plantain. Differentiation between P. infestans sporangia and powdery mildew conidia was not possible by these two methods but, when combined with analytical rules evolved by genetic programming methods, could be achieved after staining with the fluorescent brightener Calcofluor white M2R. The potential application of these techniques to the prediction of late-blight epiphytotics in the field is discussed.
AB - The ability of two different flow cytometers, the Microcyte (Optoflow) and the PAS-III (Partec), to differentiate sporangia of the late-blight pathogen Phytophthora infestans from other potential airborne particles was compared. With the PAS-III, light scatter and intrinsic fluorescence parameters could be used to differentiate sporangia from conidia of Alternaria or Botrytis spp., rust urediniospores, and pollen of grasses and plantain. Differentiation between P. infestans sporangia and powdery mildew conidia was not possible by these two methods but, when combined with analytical rules evolved by genetic programming methods, could be achieved after staining with the fluorescent brightener Calcofluor white M2R. The potential application of these techniques to the prediction of late-blight epiphytotics in the field is discussed.
U2 - 10.1128/AEM.68.1.37-45.2002
DO - 10.1128/AEM.68.1.37-45.2002
M3 - Article
SN - 1098-5336
SP - 37
EP - 45
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
ER -