Discerning combining ability loci for divergent environments using chromosome segment substitution lines (CSSLs) in pearl millet

Ramana Kumari Basava; Charles Thomas Hash; Mahesh D. Mahendarkar; Kavi Kishor P.B.; C. Tara Satyavathi; Sushil Kumar; R. B. Singh; Rattan Yadav; Rajeev Gupta; Rakesh Srivastava

doi:10.1371/journal.pone.0218916

Discerning combining ability loci for divergent environments using chromosome segment substitution lines (CSSLs) in pearl millet

Ramana Kumari Basava, Charles Thomas Hash, Mahesh D. Mahendarkar, Kavi Kishor P.B., C. Tara Satyavathi, Sushil Kumar, R. B. Singh, Rattan Yadav, Rajeev Gupta, Rakesh Srivastava

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

183 Downloads (Pure)

Abstract

Pearl millet is an important crop for arid and semi-arid regions of the world. Genomic regions associated with combining ability for yield-related traits under irrigated and drought conditions are useful in heterosis breeding programs. Chromosome segment substitution lines (CSSLs) are excellent genetic resources for precise QTL mapping and identifying naturally occurring favorable alleles. In the present study, testcross hybrid populations of 85 CSSLs were evaluated for 15 grain and stover yield-related traits for summer and wet seasons under irrigated control (CN) and moisture stress (MS) conditions. General combining ability (GCA) and specific combining ability (SCA) effects of all these traits were estimated and significant marker loci linked to GCA and SCA of the traits were identified. Heritability of the traits ranged from 53–94% in CN and 63–94% in MS. A total of 40 significant GCA loci and 36 significant SCA loci were identified for 14 different traits. Five QTLs (flowering time, panicle number and panicle yield linked to Xpsmp716 on LG4, flowering time and grain number per panicle with Xpsmp2076 on LG4) simultaneously controlled both GCA and SCA, demonstrating their unique genetic basis and usefulness for hybrid breeding programs. This study for the first time demonstrated the potential of a set of CSSLs for trait mapping in pearl millet. The novel combining ability loci linked with GCA and SCA values of the traits identified in this study may be useful in pearl millet hybrid and population improvement programs using marker-assisted selection (MAS)

Original language	English
Article number	e0218916
Journal	PLoS One
Volume	14
Issue number	8
DOIs	https://doi.org/10.1371/journal.pone.0218916
Publication status	Published - 28 Aug 2019

Keywords

GCA
SCA
CSSLs
pearl millet
Drought
Chromosomes, Plant/genetics
Chromosome Mapping/methods
Environment
Genetic Loci/genetics
Pennisetum/genetics

Access to Document

10.1371/journal.pone.0218916Licence: CC BY

Discerning combining ability loci for divergent environments using chromosome segment substitution lines (CSSLs) in pearl milletFinal published version, 1.7 MBLicence: CC BY

Permanent link

Persistent link

Cite this

@article{2265f97851f04b43af598d25f58e7509,

title = "Discerning combining ability loci for divergent environments using chromosome segment substitution lines (CSSLs) in pearl millet",

abstract = "Pearl millet is an important crop for arid and semi-arid regions of the world. Genomic regions associated with combining ability for yield-related traits under irrigated and drought conditions are useful in heterosis breeding programs. Chromosome segment substitution lines (CSSLs) are excellent genetic resources for precise QTL mapping and identifying naturally occurring favorable alleles. In the present study, testcross hybrid populations of 85 CSSLs were evaluated for 15 grain and stover yield-related traits for summer and wet seasons under irrigated control (CN) and moisture stress (MS) conditions. General combining ability (GCA) and specific combining ability (SCA) effects of all these traits were estimated and significant marker loci linked to GCA and SCA of the traits were identified. Heritability of the traits ranged from 53–94% in CN and 63–94% in MS. A total of 40 significant GCA loci and 36 significant SCA loci were identified for 14 different traits. Five QTLs (flowering time, panicle number and panicle yield linked to Xpsmp716 on LG4, flowering time and grain number per panicle with Xpsmp2076 on LG4) simultaneously controlled both GCA and SCA, demonstrating their unique genetic basis and usefulness for hybrid breeding programs. This study for the first time demonstrated the potential of a set of CSSLs for trait mapping in pearl millet. The novel combining ability loci linked with GCA and SCA values of the traits identified in this study may be useful in pearl millet hybrid and population improvement programs using marker-assisted selection (MAS)",

keywords = "GCA, SCA, CSSLs, pearl millet, Drought, Chromosomes, Plant/genetics, Chromosome Mapping/methods, Environment, Genetic Loci/genetics, Pennisetum/genetics",

author = "Basava, {Ramana Kumari} and Hash, {Charles Thomas} and Mahendarkar, {Mahesh D.} and {Kishor P.B.}, Kavi and Satyavathi, {C. Tara} and Sushil Kumar and Singh, {R. B.} and Rattan Yadav and Rajeev Gupta and Rakesh Srivastava",

note = "Funding Information: The work in part was supported by the Department of Biotechnology (DBT), Government of India, Biotechnology and Biological Sciences Research Council (BBSRC), Ministry of Science and Technology, Government of India to CTH and the Department for International Development (DFID) via grant number BB/F004133/1 and administered by CAZS Natural Resources for the benefit of developing countries. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The authors gratefully acknowledge the help of Dr. Vincent Vadez for setting up drought trials. The support of Mr. A.G. Bhaskar Raj, Mr. A. Ganapathi, Mr. Bashir, and Ms. Lanka Rama Kruti in field trials and data analysis is gratefully acknowledged. This work has been published as part of the CGIAR Research Program on Dryland Cereals, ICRISAT, India. ICRISAT is a member of the CGIAR System Organization. Publisher Copyright: {\textcopyright} 2019 Basava et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.",

year = "2019",

month = aug,

day = "28",

doi = "10.1371/journal.pone.0218916",

language = "English",

volume = "14",

journal = "PLoS One",

issn = "1932-6203",

publisher = "Public Library of Science",

number = "8",

}

TY - JOUR

T1 - Discerning combining ability loci for divergent environments using chromosome segment substitution lines (CSSLs) in pearl millet

AU - Basava, Ramana Kumari

AU - Hash, Charles Thomas

AU - Mahendarkar, Mahesh D.

AU - Kishor P.B., Kavi

AU - Satyavathi, C. Tara

AU - Kumar, Sushil

AU - Singh, R. B.

AU - Yadav, Rattan

AU - Gupta, Rajeev

AU - Srivastava, Rakesh

N1 - Funding Information: The work in part was supported by the Department of Biotechnology (DBT), Government of India, Biotechnology and Biological Sciences Research Council (BBSRC), Ministry of Science and Technology, Government of India to CTH and the Department for International Development (DFID) via grant number BB/F004133/1 and administered by CAZS Natural Resources for the benefit of developing countries. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The authors gratefully acknowledge the help of Dr. Vincent Vadez for setting up drought trials. The support of Mr. A.G. Bhaskar Raj, Mr. A. Ganapathi, Mr. Bashir, and Ms. Lanka Rama Kruti in field trials and data analysis is gratefully acknowledged. This work has been published as part of the CGIAR Research Program on Dryland Cereals, ICRISAT, India. ICRISAT is a member of the CGIAR System Organization. Publisher Copyright: © 2019 Basava et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PY - 2019/8/28

Y1 - 2019/8/28

N2 - Pearl millet is an important crop for arid and semi-arid regions of the world. Genomic regions associated with combining ability for yield-related traits under irrigated and drought conditions are useful in heterosis breeding programs. Chromosome segment substitution lines (CSSLs) are excellent genetic resources for precise QTL mapping and identifying naturally occurring favorable alleles. In the present study, testcross hybrid populations of 85 CSSLs were evaluated for 15 grain and stover yield-related traits for summer and wet seasons under irrigated control (CN) and moisture stress (MS) conditions. General combining ability (GCA) and specific combining ability (SCA) effects of all these traits were estimated and significant marker loci linked to GCA and SCA of the traits were identified. Heritability of the traits ranged from 53–94% in CN and 63–94% in MS. A total of 40 significant GCA loci and 36 significant SCA loci were identified for 14 different traits. Five QTLs (flowering time, panicle number and panicle yield linked to Xpsmp716 on LG4, flowering time and grain number per panicle with Xpsmp2076 on LG4) simultaneously controlled both GCA and SCA, demonstrating their unique genetic basis and usefulness for hybrid breeding programs. This study for the first time demonstrated the potential of a set of CSSLs for trait mapping in pearl millet. The novel combining ability loci linked with GCA and SCA values of the traits identified in this study may be useful in pearl millet hybrid and population improvement programs using marker-assisted selection (MAS)

AB - Pearl millet is an important crop for arid and semi-arid regions of the world. Genomic regions associated with combining ability for yield-related traits under irrigated and drought conditions are useful in heterosis breeding programs. Chromosome segment substitution lines (CSSLs) are excellent genetic resources for precise QTL mapping and identifying naturally occurring favorable alleles. In the present study, testcross hybrid populations of 85 CSSLs were evaluated for 15 grain and stover yield-related traits for summer and wet seasons under irrigated control (CN) and moisture stress (MS) conditions. General combining ability (GCA) and specific combining ability (SCA) effects of all these traits were estimated and significant marker loci linked to GCA and SCA of the traits were identified. Heritability of the traits ranged from 53–94% in CN and 63–94% in MS. A total of 40 significant GCA loci and 36 significant SCA loci were identified for 14 different traits. Five QTLs (flowering time, panicle number and panicle yield linked to Xpsmp716 on LG4, flowering time and grain number per panicle with Xpsmp2076 on LG4) simultaneously controlled both GCA and SCA, demonstrating their unique genetic basis and usefulness for hybrid breeding programs. This study for the first time demonstrated the potential of a set of CSSLs for trait mapping in pearl millet. The novel combining ability loci linked with GCA and SCA values of the traits identified in this study may be useful in pearl millet hybrid and population improvement programs using marker-assisted selection (MAS)

KW - GCA

KW - SCA

KW - CSSLs

KW - pearl millet

KW - Drought

KW - Chromosomes, Plant/genetics

KW - Chromosome Mapping/methods

KW - Environment

KW - Genetic Loci/genetics

KW - Pennisetum/genetics

UR - http://www.scopus.com/inward/record.url?scp=85071451089&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0218916

DO - 10.1371/journal.pone.0218916

M3 - Article

C2 - 31461465

SN - 1932-6203

VL - 14

JO - PLoS One

JF - PLoS One

IS - 8

M1 - e0218916

ER -

Discerning combining ability loci for divergent environments using chromosome segment substitution lines (CSSLs) in pearl millet

Abstract

Keywords

Access to Document

Permanent link

Other files and links

Fingerprint

Rattan Yadav

Cite this

Discerning combining ability loci for divergent environments using chromosome segment substitution lines (CSSLs) in pearl millet

Abstract

Keywords

Access to Document

Permanent link

Other files and links

Fingerprint

Profiles

Rattan Yadav

Cite this