Discriminating the ancestral progenitors of hexaploid Festuca arundinacea using genomic in situ hybridization

M.W. Humphreys; Henry M. Thomas; W. G. Morgan; M. R. Meredith; J. A. Harper; Howard Thomas; Z. Zwierzykowski; M. Ghesquiere

doi:10.1038/hdy.1995.120

Discriminating the ancestral progenitors of hexaploid Festuca arundinacea using genomic in situ hybridization

M.W. Humphreys, Henry M. Thomas, W. G. Morgan, M. R. Meredith, J. A. Harper, Howard Thomas, Z. Zwierzykowski, M. Ghesquiere

Department of Life Sciences

Institute of Grassland and Environmental Research

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104 Citations (Scopus)

Abstract

The phytogeny of Festuca arundinacea Schreb. (2n = 6x = 42) was determined using GISH. Total genomic DNA of putative ancestral species was labelled with rhodamine and hybridized to chromosome preparations of hybrids involving these species and F. arundinacea. The degree of hybridization to chromosomes known to be homologous to the probe DNA was compared with that found simultaneously on chromosomes of the genome of F. arundinacea. It was concluded that the tetraploid species Festuca arundinacea var. glaucescens contributed two genomes and the diploid species Festuca pratensis one, to create the allohexaploid species F. arundinacea.

Original language	English
Pages (from-to)	171-174
Number of pages	4
Journal	Heredity
Volume	75
Issue number	2
DOIs	https://doi.org/10.1038/hdy.1995.120
Publication status	Published - Aug 1995

Keywords

festuca arundinacea
festuca glaucescens
festuca pratensis
GISH
phylogeny
Festuca pratensis
GISH
Phytogeny
Festuca glaucescens
Festuca arundinacea

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title = "Discriminating the ancestral progenitors of hexaploid Festuca arundinacea using genomic in situ hybridization",

abstract = "The phytogeny of Festuca arundinacea Schreb. (2n = 6x = 42) was determined using GISH. Total genomic DNA of putative ancestral species was labelled with rhodamine and hybridized to chromosome preparations of hybrids involving these species and F. arundinacea. The degree of hybridization to chromosomes known to be homologous to the probe DNA was compared with that found simultaneously on chromosomes of the genome of F. arundinacea. It was concluded that the tetraploid species Festuca arundinacea var. glaucescens contributed two genomes and the diploid species Festuca pratensis one, to create the allohexaploid species F. arundinacea.",

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author = "M.W. Humphreys and Thomas, {Henry M.} and Morgan, {W. G.} and Meredith, {M. R.} and Harper, {J. A.} and Howard Thomas and Z. Zwierzykowski and M. Ghesquiere",

year = "1995",

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AU - Humphreys, M.W.

AU - Thomas, Henry M.

AU - Morgan, W. G.

AU - Meredith, M. R.

AU - Harper, J. A.

AU - Thomas, Howard

AU - Zwierzykowski, Z.

AU - Ghesquiere, M.

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KW - festuca glaucescens

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JO - Heredity

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Discriminating the ancestral progenitors of hexaploid Festuca arundinacea using genomic in situ hybridization

Abstract

Keywords

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