Distinct response kinetics of gamma interferon and interleukin-4 in bovine tuberculosis

S. G. Rhodes*, N. Palmer, S. P. Graham, A. E. Bianco, R. G. Hewinson, H. M. Vordermeier

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

52 Citations (SciVal)


This study shows that gamma interferon (IFN-γ) and interleukin-4 (IL-4) cytokine responses are produced by peripheral blood cells in cattle infected with Mycobacterium bovis. The different kinetics of the IFN-γ and IL-4 responses to bovine tuberculin and to ESAT-6 following experimental intratracheal infection with M. bovis are described. An early increase in IFN-γ was observed that was maintained throughout the period studied. In contrast, the IL-4 response was delayed and confined to a peak of activity lasting 6 to 8 weeks. Interestingly, an experimental challenge of cattle with a lower dose of M. bovis which did not result in the development of lesions, positive DTH skin test, or substantial IFN-γ responses nevertheless generated strong specific IL-4 responses. Investigation of naturally infected M. bovis field reactors showed increased IFN-γ and IL-4 responses compared to uninfected cattle and that both of these cytokines were equally able to differentiate infected from uninfected animals. The magnitude of the M. bovis-induced IL-4 responses were found to be similar to the antigen-specific IL-4 responses of cattle infected with the parasitic nematode Onchocerca ochengi, further supporting the presence of this type 2 cytokine in bovine tuberculosis.

Original languageEnglish
Pages (from-to)5393-5400
Number of pages8
JournalInfection and Immunity
Issue number9
Early online date01 Sept 2000
Publication statusPublished - 14 Sept 2000


  • Animals
  • Cattle
  • Female
  • Interferon-gamma/biosynthesis
  • Interleukin-4/biosynthesis
  • Onchocerciasis/immunology
  • Tuberculosis, Bovine/immunology


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