Distribution and activation of T-lymphocyte subsets in tuberculous bovine lymph-node granulomas

E. Liebana*, S. Marsh, J. Gough, A. Nunez, H. M. Vordermeier, A. Whelan, Y. Spencer, R. Clifton-Hardley, G. Hewinson, L. Johnson

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Citations (SciVal)


The immune response against mycobacterial infections is dependant upon a complex interaction between T lymphocytes and macrophages in the context of the granuloma. For this study, we performed the analysis of 18 stage I or II, and 13 stage III or IV granulomas found in lymph nodes from 8 experimentally and 2 naturally infected cattle. T-cell subpopulations (CD3+, CD4 +, CD8+, WC1+, CD25+) were investigated by immunohistochemistry. In the majority of stage I/II lesions, CD8+ and CD25+ cells were predominantly found in the lymphocytic outer region of the granuloma, suggesting a possible role for activated CD8+ cells in the initial attempt to restrain the granuloma growth. CD4+ T cells appeared equally distributed in the lymphocytic mantle and in the internal areas of the granulomas. WC1+ cells appeared interspersed among the macrophages. We speculated that this could indicate a role for these 2 subsets in the maintenance and the maturation of the granuloma. In stage III/IV lesions, all of the T-cell subsets investigated appeared interspersed among the mononuclear component of the granulomas. In general terms, there was a higher density of CD8+ cells compared with CD4+ cells. However, there was no sense of rimming effect for any of the investigated cell populations.

Original languageEnglish
Pages (from-to)366-372
Number of pages7
JournalVeterinary Pathology
Issue number3
Publication statusPublished - 01 May 2007


  • Bovine
  • Immunopathology
  • T-cells
  • Tuberculosis


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