DNA extraction from spider webs

Max Blake, Niall McKeown, Mark Bushell, Paul Shaw

Research output: Contribution to journalArticlepeer-review

8 Citations (SciVal)
153 Downloads (Pure)

Abstract

Many spider species produce webs that represent a potential non-invasive source of DNA for conservation genetic analysis. Reported here is the successful isolation of target DNA from members of two families (Theraphosidae and Pholcidae) using a standard CTAB phenol–chloroform–isoamyl protocol. The isolated DNA was of sufficient quality to permit routine PCR amplification and sequencing of mtDNA COI fragments of various sizes (maximum 710 bp attempted). This adds to other studies in demonstrating that webbing offers an excellent resource for genetic studies of spiders across families. Applications of the technique include species identification and monitoring, faunistic surveys, population connectivity, subpopulation structuring, and ex situ breeding programs.
Original languageEnglish
Pages (from-to)219-221
Number of pages3
JournalConservation Genetics Resources
Volume8
Issue number3
Early online date26 Apr 2016
DOIs
Publication statusPublished - 01 Sept 2016

Keywords

  • spider webs
  • ecological genetics
  • non-destructive sampling
  • non-invasive samplint
  • conservation
  • psalmopoeus
  • pholcus

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