Early hepatic and peritoneal changes and immune response in goats vaccinated with a recombinant glutathione transferase sigma class and challenged with Fasciola hepatica.

R Zafra, R. A. Pérez-Écija, L. Buffoni, I. L. Pacheco, A. Martínez-Moreno, E. J. Lacourse , Samirah Perally, Peter Michael Brophy, J. Pérez

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27 Citations (SciVal)

Abstract

Changes and local immune response were evaluated in the peritoneal cell populations, duodenal lamina propria and liver from goats immunized with recombinant glutathione transferase sigma class (rFhGST-S1) during early stages of infection with Fasciola hepatica. Group 1 (n=7) was unimmunized and uninfected; group 2 (n=10) was immunized with adjuvant Quil A and infected; group 3 (n=10) was immunised with rFhGST-S1 and infected. Three goats from each group were killed at 7-9days post-infection (dpi) to evaluate early changes and immune response. The remaining goats were killed at 15weeks post-infection (wpi). rFhGST-S1 vaccination induced variable response: three goats showed low fluke burden at 15 wpi and two goats showed low hepatic damage at early infection stages. This response was associated to a severe infiltrate of eosinophils in peritoneal fluid and hepatic necrotic foci, high iNOS expression in peritoneal cells and abundant infiltrate of eosinophils surrounding hepatic migrating flukes. T lymphocyte subsets were found in the vicinity of necrotic areas but they were absent in the vicinity of migrating larvae. No significant variation for T cell subsets, except for CD4 and γδ T lymphocytes, that were higher in the Quil A group compared to the rFhGST-S1 group. Expression of IL4 and IFN-γ in the hepatic inflammatory infiltrates was very occasional
Original languageEnglish
Pages (from-to)602-609
Number of pages8
JournalResearch in Veterinary Science
Volume94
Issue number3
Early online date20 Dec 2012
DOIs
Publication statusPublished - 01 Jun 2013

Keywords

  • Fasciola hepatica
  • Glutathione S-transferase
  • vaccine
  • goats

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