Effect of abomasal infusions of geometric isomers of 10,12 conjugated linoleic acid on milk fat synthesis in dairy cows

Asgeir Saebø, Per-Christian Saebø, J Mikko Griinari, Kevin J Shingfield

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75 Citations (SciVal)


The trans-10,cis-12 isomer of conjugated linoleic acid (CLA) decreases TAG accumulation in 3T3-L1 adipocytes, reduces lipid accretion in growing animals, and inhibits milk fat synthesis in lactating mammals. However, there is evidence to suggest that other FA may also exert antilipogenic effects. In the current experiment, the effects of geometric isomers of 10,12 CLA on milk fat synthesis were examined using four Holstein-British Friesian cows in a 4 x 4 Latin Square experiment with 14-d periods. Treatments consisted of abomasal infusions of skim milk, or skim milk containing trans-10,cis-12 CLA (T1), trans-10,trans-12 CLA (T2), or a mixture of predominantly 10,12 isomers containing (g/l00 g) trans-10,cis-12 (35.0), cis-10,trans-12 (23.2), trans-10,trans-12 (14.9), and cis-10,cis-12 (5.1). CLA supplements were prepared from purified ethyl linoleate and infused as nonesterified FA. Infusions were conducted over a 4-d period with a 10-d interval between treatments and targeted to deliver 4.5 g/d of 10,12 CLA isomers. Compared with the control, trans-10, trans-12 CLA had no effect (P> 0.05) on milk fat yield, whereas treatments T1 and T3 depressed (P <0.05) milk fat content (19.8 and 22.9%, respectively) and decreased milk fat output (20.8 and 21.3%, respectively). Comparable reductions in milk fat synthesis to 4.14 and 1.80 g trans-10,cis-12/d supplied by treatments T1 and T3 indicate that other 10,12 geometric isomers of CLA have the potential to exert antilipogenic effects. The relative abundance of cis-10,trans-12 CLA in treatment T3 and the low transfer efficiency of this isomer into milk suggest that cis-10,trans-12 CLA was the active component..
Original languageEnglish
Pages (from-to)823-32
Number of pages10
Issue number8
Publication statusPublished - Aug 2005


  • Animals
  • Cattle
  • Chromatography, Gas
  • Chromatography, High Pressure Liquid
  • Dietary Fats
  • Esters
  • Female
  • Isomerism
  • Linoleic Acids, Conjugated
  • Milk


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