Effect of dose and route of immunisation on the immune response induced in cattle by heterologous Bacille Calmette-Guerin priming and recombinant adenoviral vector boosting

G. Dean; D. Clifford; S. Gilbert; H. McShane; R. G. Hewinson; H. M. Vordermeier; B. Villarreal-Ramos

doi:10.1016/j.vetimm.2014.01.010

Effect of dose and route of immunisation on the immune response induced in cattle by heterologous Bacille Calmette-Guerin priming and recombinant adenoviral vector boosting

G. Dean, D. Clifford, S. Gilbert, H. McShane, R. G. Hewinson, H. M. Vordermeier, B. Villarreal-Ramos

Bovine TB, diseases and control

Research output: Contribution to journal › Article › peer-review

Abstract

BCG is used experimentally as a vaccine against tuberculosis (TB), induced by Mycobacterium bovis, in cattle (bTB). However, the efficacy of BCG is variable in humans, cattle and guinea pigs. An adenoviral vector expressing Antigen 85A (Ad5Ag85A) has enhanced protection against TB in mice when used in combination with BCG for prime-boost experiments. However, the route of immunisation affects the degree of protection seen. This work examines the immunogenicity of a new vectored vaccine (Ad5-TBF) that expresses Ag85A, Rv0287, Rv0288 and Rv0251c to explore the effects of dose of adenoviral boost and route of inoculation on immunogenicity. We found that 2×10⁹ infectious units (iu) delivered intradermally conferred the most consistent and strongest responses of the different regimes tested.

Original language	English
Pages (from-to)	208-213
Number of pages	6
Journal	Veterinary Immunology and Immunopathology
Volume	158
Issue number	3-4
Early online date	28 Jan 2014
DOIs	https://doi.org/10.1016/j.vetimm.2014.01.010
Publication status	Published - 15 Apr 2014

Keywords

Adenovirus
Mycobacteria
Route of immunisation
Tuberculosis
Vaccine

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.vetimm.2014.01.010

Permanent link

Persistent link

Cite this

Dean, G., Clifford, D., Gilbert, S., McShane, H., Hewinson, R. G., Vordermeier, H. M., & Villarreal-Ramos, B. (2014). Effect of dose and route of immunisation on the immune response induced in cattle by heterologous Bacille Calmette-Guerin priming and recombinant adenoviral vector boosting. Veterinary Immunology and Immunopathology, 158(3-4), 208-213. https://doi.org/10.1016/j.vetimm.2014.01.010

@article{f1c85d49f83a4fb283cc8c4e67c8f8a4,

title = "Effect of dose and route of immunisation on the immune response induced in cattle by heterologous Bacille Calmette-Guerin priming and recombinant adenoviral vector boosting",

abstract = "BCG is used experimentally as a vaccine against tuberculosis (TB), induced by Mycobacterium bovis, in cattle (bTB). However, the efficacy of BCG is variable in humans, cattle and guinea pigs. An adenoviral vector expressing Antigen 85A (Ad5Ag85A) has enhanced protection against TB in mice when used in combination with BCG for prime-boost experiments. However, the route of immunisation affects the degree of protection seen. This work examines the immunogenicity of a new vectored vaccine (Ad5-TBF) that expresses Ag85A, Rv0287, Rv0288 and Rv0251c to explore the effects of dose of adenoviral boost and route of inoculation on immunogenicity. We found that 2×109 infectious units (iu) delivered intradermally conferred the most consistent and strongest responses of the different regimes tested.",

keywords = "Adenovirus, Mycobacteria, Route of immunisation, Tuberculosis, Vaccine",

author = "G. Dean and D. Clifford and S. Gilbert and H. McShane and Hewinson, {R. G.} and Vordermeier, {H. M.} and B. Villarreal-Ramos",

year = "2014",

month = apr,

day = "15",

doi = "10.1016/j.vetimm.2014.01.010",

language = "English",

volume = "158",

pages = "208--213",

journal = "Veterinary Immunology and Immunopathology",

issn = "0165-2427",

publisher = "Elsevier",

number = "3-4",

}

Dean, G, Clifford, D, Gilbert, S, McShane, H, Hewinson, RG, Vordermeier, HM & Villarreal-Ramos, B 2014, 'Effect of dose and route of immunisation on the immune response induced in cattle by heterologous Bacille Calmette-Guerin priming and recombinant adenoviral vector boosting', Veterinary Immunology and Immunopathology, vol. 158, no. 3-4, pp. 208-213. https://doi.org/10.1016/j.vetimm.2014.01.010

Effect of dose and route of immunisation on the immune response induced in cattle by heterologous Bacille Calmette-Guerin priming and recombinant adenoviral vector boosting. / Dean, G.; Clifford, D.; Gilbert, S. et al.

In: Veterinary Immunology and Immunopathology, Vol. 158, No. 3-4, 15.04.2014, p. 208-213.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Effect of dose and route of immunisation on the immune response induced in cattle by heterologous Bacille Calmette-Guerin priming and recombinant adenoviral vector boosting

AU - Dean, G.

AU - Clifford, D.

AU - Gilbert, S.

AU - McShane, H.

AU - Hewinson, R. G.

AU - Vordermeier, H. M.

AU - Villarreal-Ramos, B.

PY - 2014/4/15

Y1 - 2014/4/15

N2 - BCG is used experimentally as a vaccine against tuberculosis (TB), induced by Mycobacterium bovis, in cattle (bTB). However, the efficacy of BCG is variable in humans, cattle and guinea pigs. An adenoviral vector expressing Antigen 85A (Ad5Ag85A) has enhanced protection against TB in mice when used in combination with BCG for prime-boost experiments. However, the route of immunisation affects the degree of protection seen. This work examines the immunogenicity of a new vectored vaccine (Ad5-TBF) that expresses Ag85A, Rv0287, Rv0288 and Rv0251c to explore the effects of dose of adenoviral boost and route of inoculation on immunogenicity. We found that 2×109 infectious units (iu) delivered intradermally conferred the most consistent and strongest responses of the different regimes tested.

AB - BCG is used experimentally as a vaccine against tuberculosis (TB), induced by Mycobacterium bovis, in cattle (bTB). However, the efficacy of BCG is variable in humans, cattle and guinea pigs. An adenoviral vector expressing Antigen 85A (Ad5Ag85A) has enhanced protection against TB in mice when used in combination with BCG for prime-boost experiments. However, the route of immunisation affects the degree of protection seen. This work examines the immunogenicity of a new vectored vaccine (Ad5-TBF) that expresses Ag85A, Rv0287, Rv0288 and Rv0251c to explore the effects of dose of adenoviral boost and route of inoculation on immunogenicity. We found that 2×109 infectious units (iu) delivered intradermally conferred the most consistent and strongest responses of the different regimes tested.

KW - Adenovirus

KW - Mycobacteria

KW - Route of immunisation

KW - Tuberculosis

KW - Vaccine

UR - http://www.scopus.com/inward/record.url?scp=84897097199&partnerID=8YFLogxK

U2 - 10.1016/j.vetimm.2014.01.010

DO - 10.1016/j.vetimm.2014.01.010

M3 - Article

C2 - 24581917

AN - SCOPUS:84897097199

SN - 0165-2427

VL - 158

SP - 208

EP - 213

JO - Veterinary Immunology and Immunopathology

JF - Veterinary Immunology and Immunopathology

IS - 3-4

ER -

Dean G, Clifford D, Gilbert S, McShane H, Hewinson RG, Vordermeier HM et al. Effect of dose and route of immunisation on the immune response induced in cattle by heterologous Bacille Calmette-Guerin priming and recombinant adenoviral vector boosting. Veterinary Immunology and Immunopathology. 2014 Apr 15;158(3-4):208-213. Epub 2014 Jan 28. doi: 10.1016/j.vetimm.2014.01.010

Effect of dose and route of immunisation on the immune response induced in cattle by heterologous Bacille Calmette-Guerin priming and recombinant adenoviral vector boosting

Abstract

Keywords

UN SDGs

Access to Document

Permanent link

Other files and links

Fingerprint

Cite this