TY - JOUR
T1 - Effect of Peripheral 5-HT on Glucose and Lipid Metabolism in Wether Sheep
AU - Watanabe, Hitoshi
AU - Saito, Ryo
AU - Nakano, Tatsuya
AU - Takahashi, Hideyuki
AU - Takahashi, Yu
AU - Sumiyoshi, Keisuke
AU - Sato, Katsuyoshi
AU - Chen, Xiangning
AU - Okada, Natsumi
AU - Iwasaki, Shunsuke
AU - Harjanti, Dian W.
AU - Sekiguchi, Natsumi
AU - Sano, Hiroaki
AU - Kitazawa, Haruki
AU - Rose, Michael T.
AU - Ohwada, Shyuichi
AU - Watanabe, Kouichi
AU - Aso, Hisashi
A2 - Vaudry, Hubert
N1 - Watanabe, H., Saito, R., Nakano, T., Takahashi, H., Takahashi, Y., Sumiyoshi, K., Sato, K., Chen, X., Okada, N., Iwasaki, S., Harjanti, D. W., Sekiguchi, N., Sano, H., Kitazawa, H., Rose, M. T., Ohwada, S., Watanabe, K., Aso, H. & Vaudry, H. (Ed.) (2014). Effect of Peripheral 5-HT on Glucose and Lipid Metabolism in Wether Sheep. PLoS One, 9 (2), [e88058]
PY - 2014/2/4
Y1 - 2014/2/4
N2 - In mice, peripheral 5-HT induces an increase in the plasma concentrations of glucose, insulin and bile acids, and a decrease in plasma triglyceride, NEFA and cholesterol concentrations. However, given the unique characteristics of the metabolism of ruminants relative to monogastric animals, the physiological role of peripheral 5-HT on glucose and lipid metabolism in sheep remains to be established. Therefore, in this study, we investigated the effect of 5-HT on the circulating concentrations of metabolites and insulin using five 5-HT receptor (5HTR) antagonists in sheep. After fasting for 24 h, sheep were intravenously injected with 5-HT, following which-, plasma glucose, insulin, triglyceride and NEFA concentrations were significantly elevated. In contrast, 5-HT did not affect the plasma cholesterol concentration, and it induced a decrease in bile acid concentrations. Increases in plasma glucose and insulin concentrations induced by 5-HT were attenuated by pre-treatment with Methysergide, a 5HTR 1, 2 and 7 antagonist. Additionally, decreased plasma bile acid concentrations induced by 5-HT were blocked by pre-treatment with Ketanserin, a 5HTR 2A antagonist. However, none of the 5HTR antagonists inhibited the increase in plasma triglyceride and NEFA levels induced by 5-HT. On the other hand, mRNA expressions of 5HTR1D and 1E were observed in the liver, pancreas and skeletal muscle. These results suggest that there are a number of differences in the physiological functions of peripheral 5-HT with respect to lipid metabolism between mice and sheep, though its effect on glucose metabolism appears to be similar between these species.
AB - In mice, peripheral 5-HT induces an increase in the plasma concentrations of glucose, insulin and bile acids, and a decrease in plasma triglyceride, NEFA and cholesterol concentrations. However, given the unique characteristics of the metabolism of ruminants relative to monogastric animals, the physiological role of peripheral 5-HT on glucose and lipid metabolism in sheep remains to be established. Therefore, in this study, we investigated the effect of 5-HT on the circulating concentrations of metabolites and insulin using five 5-HT receptor (5HTR) antagonists in sheep. After fasting for 24 h, sheep were intravenously injected with 5-HT, following which-, plasma glucose, insulin, triglyceride and NEFA concentrations were significantly elevated. In contrast, 5-HT did not affect the plasma cholesterol concentration, and it induced a decrease in bile acid concentrations. Increases in plasma glucose and insulin concentrations induced by 5-HT were attenuated by pre-treatment with Methysergide, a 5HTR 1, 2 and 7 antagonist. Additionally, decreased plasma bile acid concentrations induced by 5-HT were blocked by pre-treatment with Ketanserin, a 5HTR 2A antagonist. However, none of the 5HTR antagonists inhibited the increase in plasma triglyceride and NEFA levels induced by 5-HT. On the other hand, mRNA expressions of 5HTR1D and 1E were observed in the liver, pancreas and skeletal muscle. These results suggest that there are a number of differences in the physiological functions of peripheral 5-HT with respect to lipid metabolism between mice and sheep, though its effect on glucose metabolism appears to be similar between these species.
UR - http://hdl.handle.net/2160/26347
U2 - 10.1371/journal.pone.0088058
DO - 10.1371/journal.pone.0088058
M3 - Article
C2 - 24505376
SN - 1932-6203
VL - 9
JO - PLoS ONE
JF - PLoS ONE
IS - 2
M1 - e88058
ER -