Effect of variation in the proportion of solid- and liquid-associated rumen bacteria in duodenal contents on the estimation of duodenal bacterial nitrogen flow

Richard J. Dewhurst, B. Vlaeminck, D. Demeyer, V. Fievez

Research output: Contribution to journalArticlepeer-review

4 Citations (SciVal)

Abstract

The aim of this research was to examine to what extent variation in the relative proportions of solid- (SAB) and liquid-associated rumen bacteria (LAB) in duodenal bacteria have an impact on the estimation of duodenal flowofbacterialN.Forthis,fourdairycowswerefeddietsvaryinginforage:concentrate ratio (80:20, 65:35, 50:50 and 35:65). SAB and LAB were separated from rumen contents four h after the morning feeding. Adenine, cytosine and odd and branched-chain fatty acids were determined both in SAB and LAB and used to estimate bacterial N flow. Bacterial N flowswerealsocalculated using a SAB:LAB ratio in duodenal bacteria, as estimated from the odd and branched-chain fatty acid pattern. Compared with calculations based on the estimated SAB:LAB ratio, estimations based on SAB or LAB only as a bacterial reference on average over- and underestimated bacterial N flowby37and55gN/d,respectively(P
Original languageEnglish
Pages (from-to)37-42
Number of pages6
JournalJournal of Animal and Feed Sciences
Publication statusPublished - 2007

Fingerprint

Dive into the research topics of 'Effect of variation in the proportion of solid- and liquid-associated rumen bacteria in duodenal contents on the estimation of duodenal bacterial nitrogen flow'. Together they form a unique fingerprint.

Cite this