Efficient method for rapid multiplication of clean and healthy willow clones via in vitro propagation with broad genotype applicability

Elena Palomo-Rios, William Macalpine, Ian Shield, Joanna Amey, Cuma Karaoglu, Jevon West, Steven Hanley, Richard Krygier, Angela Karp, Huw D Jones

Research output: Contribution to journalArticlepeer-review

Abstract

Willow is a versatile crop with considerable potential as a source of renewable biomass for bioenergy. Although breeding new varieties takes less time compared with some other tree species, producing new willow varieties is still a slow, labour-intensive process, partly because clonally propagating the results of each cross is a bottleneck early in the breeding scheme. In this paper, we describe a facile, rapid method for the in vitro culture of a wide range of willow genotypes. We have developed a combination of media and methods for efficient tissue-culture propagation to rapidly multiply individual plants and simultaneously produce clean, stock germplasm applicable to a wide range of willow genotypes that can be phytosanitary tested to demonstrate their disease-free status. The micropropagation method described could generate in the order of 5000 viable, transplantable clones from a single plant in just 24 weeks and was used to produce phytosanitary tested breeding material for export to overcome restriction on the international transport of woody cuttings. This method could represent a valuable biotechnology adjunct to willow breeding programmes and could accommodate early selection via molecular or biochemical markers.

Original languageEnglish
Pages (from-to)1662-1667
Number of pages6
JournalCanadian Journal of Forest Research
Volume45
Issue number11
DOIs
Publication statusPublished - 01 Nov 2015
Externally publishedYes

Keywords

  • willow breeding (Salix)
  • in vitro culture
  • multiplication
  • propagation
  • phytosanitary
  • SALIX
  • MICROPROPAGATION
  • IMPROVEMENT
  • BIOENERGY
  • PLANTS
  • MEDIA

Fingerprint

Dive into the research topics of 'Efficient method for rapid multiplication of clean and healthy willow clones via in vitro propagation with broad genotype applicability'. Together they form a unique fingerprint.

Cite this