Electrophysiological analysis of responses of adult females of Brugia pahangi to some chemicals

Roland N. Perry; John Barrett; R. N. Rolfe

doi:10.1017/S0031182001007375

Electrophysiological analysis of responses of adult females of Brugia pahangi to some chemicals

Roland N. Perry, John Barrett, R. N. Rolfe

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Electrophysiological techniques were used to obtain recordings of extracellular electrical activity from the anterior end of live adult females of Brugia pahangi. Stimulation with 100mM, 10mM and 1mM acetylcholine resulted in an increase in spike activity which was concentration dependent, whereas stimulation with phosphate-buffered saline and 0·1mM acetylcholine gave no increase in activity. The delay in response was not concentration dependent. The action of possible host cues was investigated. Stimulation with heat-inactivated foetal calf serum (IFCS) and 10mM glutathione gave an increase in spike activity but exposure to 5mg/dl haemoglobin elicited no response. The response to IFCS was found to be suppressed completely by pre-incubation for 30min in ivermectin.

Original language	English
Pages (from-to)	347-357
Number of pages	11
Journal	Parasitology
Volume	122
Issue number	3
DOIs	https://doi.org/10.1017/S0031182001007375
Publication status	Published - 2001

Keywords

Animal-parasitic nematodes
Electrophysiology
Extracellular responses
Sensilla
Sensory perception

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10.1017/S0031182001007375

http://hdl.handle.net/2160/3582

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title = "Electrophysiological analysis of responses of adult females of Brugia pahangi to some chemicals",

abstract = "Electrophysiological techniques were used to obtain recordings of extracellular electrical activity from the anterior end of live adult females of Brugia pahangi. Stimulation with 100mM, 10mM and 1mM acetylcholine resulted in an increase in spike activity which was concentration dependent, whereas stimulation with phosphate-buffered saline and 0·1mM acetylcholine gave no increase in activity. The delay in response was not concentration dependent. The action of possible host cues was investigated. Stimulation with heat-inactivated foetal calf serum (IFCS) and 10mM glutathione gave an increase in spike activity but exposure to 5mg/dl haemoglobin elicited no response. The response to IFCS was found to be suppressed completely by pre-incubation for 30min in ivermectin.",

keywords = "Animal-parasitic nematodes, Electrophysiology, Extracellular responses, Sensilla, Sensory perception",

author = "Perry, {Roland N.} and John Barrett and Rolfe, {R. N.}",

note = "Rolfe, R. N., Barrett, J., Perry, R. N. (2001). Electrophysiological analysis of responses of adult females of Brugia pahangi to some chemicals. Parasitology, 122,(3), 347-357. Sponsorship: BBSRC",

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T1 - Electrophysiological analysis of responses of adult females of Brugia pahangi to some chemicals

AU - Perry, Roland N.

AU - Barrett, John

AU - Rolfe, R. N.

N1 - Rolfe, R. N., Barrett, J., Perry, R. N. (2001). Electrophysiological analysis of responses of adult females of Brugia pahangi to some chemicals. Parasitology, 122,(3), 347-357. Sponsorship: BBSRC

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N2 - Electrophysiological techniques were used to obtain recordings of extracellular electrical activity from the anterior end of live adult females of Brugia pahangi. Stimulation with 100mM, 10mM and 1mM acetylcholine resulted in an increase in spike activity which was concentration dependent, whereas stimulation with phosphate-buffered saline and 0·1mM acetylcholine gave no increase in activity. The delay in response was not concentration dependent. The action of possible host cues was investigated. Stimulation with heat-inactivated foetal calf serum (IFCS) and 10mM glutathione gave an increase in spike activity but exposure to 5mg/dl haemoglobin elicited no response. The response to IFCS was found to be suppressed completely by pre-incubation for 30min in ivermectin.

AB - Electrophysiological techniques were used to obtain recordings of extracellular electrical activity from the anterior end of live adult females of Brugia pahangi. Stimulation with 100mM, 10mM and 1mM acetylcholine resulted in an increase in spike activity which was concentration dependent, whereas stimulation with phosphate-buffered saline and 0·1mM acetylcholine gave no increase in activity. The delay in response was not concentration dependent. The action of possible host cues was investigated. Stimulation with heat-inactivated foetal calf serum (IFCS) and 10mM glutathione gave an increase in spike activity but exposure to 5mg/dl haemoglobin elicited no response. The response to IFCS was found to be suppressed completely by pre-incubation for 30min in ivermectin.

KW - Animal-parasitic nematodes

KW - Electrophysiology

KW - Extracellular responses

KW - Sensilla

KW - Sensory perception

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DO - 10.1017/S0031182001007375

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EP - 357

JO - Parasitology

JF - Parasitology

IS - 3

ER -

Electrophysiological analysis of responses of adult females of Brugia pahangi to some chemicals

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Keywords

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