@inbook{827b1b4716874c8a8c4a4ee313ce37b9,
title = "ELISPOT assays to enumerate bovine IFN-γ-secreting cells for the development of novel vaccines against bovine tuberculosis",
abstract = "Enumeration of antigen-specific cells after vaccination is one of the prime immunological parameters determined when developing vaccines. Due to their exquisite sensitivity (limits of detection can be below 1/100,000 cells), ELISPOT assays are therefore an important tool in vaccine development programs. This is particularly the case for vaccines against diseases that require protective cell-mediated immunity, such as tuberculosis. This chapter describes ELISPOT assays detecting bovine IFN-γ.",
keywords = "Cultured ELISPOT, Cytokines, Effector and memory T-cell responses, ELISPOT, IFN-γ",
author = "Martin Vordermeier and Whelan, {Adam O.}",
year = "2012",
month = jan,
day = "1",
doi = "10.1007/978-1-61779-325-7_17",
language = "English",
isbn = "9781617793240",
series = "Methods in Molecular Biology",
pages = "219--227",
editor = "Alexander Kalyuzhny",
booktitle = "Handbook of ELISPOT",
}
