ELISPOT assays to enumerate bovine IFN-γ-secreting cells for the development of novel vaccines against bovine tuberculosis

Martin Vordermeier*, Adam O. Whelan

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

10 Citations (SciVal)

Abstract

Enumeration of antigen-specific cells after vaccination is one of the prime immunological parameters determined when developing vaccines. Due to their exquisite sensitivity (limits of detection can be below 1/100,000 cells), ELISPOT assays are therefore an important tool in vaccine development programs. This is particularly the case for vaccines against diseases that require protective cell-mediated immunity, such as tuberculosis. This chapter describes ELISPOT assays detecting bovine IFN-γ.

Original languageEnglish
Title of host publicationHandbook of ELISPOT
Subtitle of host publicationMethods and Protocols
EditorsAlexander Kalyuzhny
Pages219-227
Number of pages9
DOIs
Publication statusPublished - 01 Jan 2012

Publication series

NameMethods in Molecular Biology
Volume792
ISSN (Print)1064-3745

Keywords

  • Cultured ELISPOT
  • Cytokines
  • Effector and memory T-cell responses
  • ELISPOT
  • IFN-γ

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