ENDOSPERM DEFECTIVE1 Is a Novel Microtubule-Associated Protein Essential for Seed Development in Arabidopsis

Cristina Pignocchi, Gregory E Minns, Nathalie Nesi, Rachil Koumproglou, Georgios Kitsios, Christoph Benning, Clive W Lloyd, John Hugh Doonan, Matthew J. Hills

Research output: Contribution to journalArticlepeer-review

67 Citations (SciVal)

Abstract

Early endosperm development involves a series of rapid nuclear divisions in the absence of cytokinesis; thus, many endosperm mutants reveal genes whose functions are essential for mitosis. This work finds that the endosperm of Arabidopsis thaliana endosperm-defective1 (ede1) mutants never cellularizes, contains a reduced number of enlarged polyploid nuclei, and features an aberrant microtubule cytoskeleton, where the specialized radial microtubule systems and cytokinetic phragmoplasts are absent. Early embryo development is substantially normal, although occasional cytokinesis defects are observed. The EDE1 gene was cloned using a map-based approach and represents the pioneer member of a conserved plant-specific family of genes of previously unknown function. EDE1 is expressed in the endosperm and embryo of developing seeds, and its expression is tightly regulated during cell cycle progression. EDE1 protein accumulates in nuclear caps in premitotic cells, colocalizes along microtubules of the spindle and phragmoplast, and binds microtubules in vitro. We conclude that EDE1 is a novel plant-specific microtubule-associated protein essential for microtubule function during the mitotic and cytokinetic stages that generate the Arabidopsis endosperm and embryo.
Original languageEnglish
Pages (from-to)90-105
Number of pages16
JournalPlant Cell
Volume21
Issue number1
Early online date16 Jan 2009
DOIs
Publication statusPublished - 21 Jan 2009

Fingerprint

Dive into the research topics of 'ENDOSPERM DEFECTIVE1 Is a Novel Microtubule-Associated Protein Essential for Seed Development in Arabidopsis'. Together they form a unique fingerprint.

Cite this