Real-time PCR was used to detect and quantify Mycobacterium bovis cells in naturally infected soil and badger feces. Immunomagnetic capture, immunofluorescence, and selective culture confirmed species identification and cell viability. These techniques will prove useful for monitoring M. bovis in the environment and for elucidating transmission routes between wildlife and cattle.
|Number of pages||3|
|Journal||Applied and Environmental Microbiology|
|Early online date||28 Sept 2007|
|Publication status||Published - 01 Nov 2007|