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Enzyme combinations producing short-chain cello-oligosaccharides (COS) as major bio-products from cellulose of Miscanthus Mx2779 accessed through different pretreatment methods were compared. Over short hydrolysis times, processive endoglucanase TfCel9a produced a high percentage of cellotetraose and cellopentaose and is synergistic with endoglucanase CcCel9m for producing short oligomers from amorphous cellulose but had low activity on untreated Miscanthus. Hydrolysis of the latter improved when these were combined with a mutant cellobio/triohydrolase OsCelC7(−105) and a lytic polysaccharide monooxygenase TrCel61a, a combination which also produced the highest COS yields from phosphoric acid swollen cellulose. Steam explosion pretreatment of Miscanthus increased COS yields, with/without phosphoric acid swelling, while increased swelling time (from 20 to 45 min) also increased yields but decreased the need for TrCel61a. The highest COS yields (933 mg/g glucan) and most stable product profile were obtained using ionic liquid [C2mim][OAc] pretreatment and the three enzyme mixture TfCel9a, Cel9m and OsCel7a(−105).
|Number of pages||12|
|Early online date||01 Jun 2022|
|Publication status||Published - 01 Aug 2022|
- Enzymatic hydrolysis
- Lytic polysaccharide monooxygenase
- CELLULOSE CRYSTALLINE-STRUCTURE
- IONIC LIQUID PRETREATMENT
- CORN STOVER
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- 1 Finished
BEACON- EAST Wales
01 Jul 2019 → 30 Jun 2022
Project: Externally funded research