Evaluation of a membrane filtration method for the rapid enumeration of confirmed Clostridium perfringens from water

J. Watkins; D. P. Sartory

doi:10.1111/lam.12383

Evaluation of a membrane filtration method for the rapid enumeration of confirmed Clostridium perfringens from water

J. Watkins, D. P. Sartory

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

A modification of the UK reference and ISO 14189 TSCA medium for the enumeration of Clostridium perfringens from water coupled with a membrane filter transfer technique for testing for production of acid phosphatase was evaluated. The new tryptose cycloserine agar (TCA) medium, which lacks sodium metabisulphite but contains sodium pyruvate to improve recovery, allows the isolation and confirmation of Cl. perfringens within 18–24 h of sample processing. Data from a multilaboratory study analysed according to ISO 17994 showed that TCA was equivalent to TSCA for the enumeration of Cl. perfringens. The identification of acid phosphatase-negative isolates revealed a false-negative rate for the TCA method of 0·8%. The TCA membrane filter transfer procedure provides confirmed Cl. perfringens counts in half the time of the TSCA method and is simple to undertake.

Original language	English
Pages (from-to)	367-371
Number of pages	5
Journal	Letters in Applied Microbiology
Volume	60
Issue number	4
DOIs	https://doi.org/10.1111/lam.12383
Publication status	Published - 15 Mar 2015
Externally published	Yes

Keywords

Clostridium perfringens
enumeration
indicators
rapid methods
water quality

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title = "Evaluation of a membrane filtration method for the rapid enumeration of confirmed Clostridium perfringens from water",

abstract = "A modification of the UK reference and ISO 14189 TSCA medium for the enumeration of Clostridium perfringens from water coupled with a membrane filter transfer technique for testing for production of acid phosphatase was evaluated. The new tryptose cycloserine agar (TCA) medium, which lacks sodium metabisulphite but contains sodium pyruvate to improve recovery, allows the isolation and confirmation of Cl. perfringens within 18–24 h of sample processing. Data from a multilaboratory study analysed according to ISO 17994 showed that TCA was equivalent to TSCA for the enumeration of Cl. perfringens. The identification of acid phosphatase-negative isolates revealed a false-negative rate for the TCA method of 0·8%. The TCA membrane filter transfer procedure provides confirmed Cl. perfringens counts in half the time of the TSCA method and is simple to undertake.",

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AU - Sartory, D. P.

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AB - A modification of the UK reference and ISO 14189 TSCA medium for the enumeration of Clostridium perfringens from water coupled with a membrane filter transfer technique for testing for production of acid phosphatase was evaluated. The new tryptose cycloserine agar (TCA) medium, which lacks sodium metabisulphite but contains sodium pyruvate to improve recovery, allows the isolation and confirmation of Cl. perfringens within 18–24 h of sample processing. Data from a multilaboratory study analysed according to ISO 17994 showed that TCA was equivalent to TSCA for the enumeration of Cl. perfringens. The identification of acid phosphatase-negative isolates revealed a false-negative rate for the TCA method of 0·8%. The TCA membrane filter transfer procedure provides confirmed Cl. perfringens counts in half the time of the TSCA method and is simple to undertake.

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KW - indicators

KW - rapid methods

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Evaluation of a membrane filtration method for the rapid enumeration of confirmed Clostridium perfringens from water

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Keywords

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