Evaluation of a modified semi-automated immunomagnetic separation technique for the detection of Cryptosporidium oocysts in human faeces

G. Robinson; J. Watkins; R. M. Chalmers

doi:10.1016/j.mimet.2008.04.006

Evaluation of a modified semi-automated immunomagnetic separation technique for the detection of Cryptosporidium oocysts in human faeces

G. Robinson, J. Watkins, R. M. Chalmers

Research output: Contribution to journal › Article › peer-review

19 Citations (SciVal)

Abstract

Routine diagnosis of Cryptosporidium infection relies on the oocyst as the diagnostic target. To improve analytical sensitivity, we modified and evaluated an immunomagnetic separation procedure for Cryptosporidium oocysts applicable to formed, liquid or mucoid human faeces. The analytical sensitivity of the method is < 5 oocysts per gram, regardless of stool consistency.

Original language	English
Pages (from-to)	139-141
Number of pages	3
Journal	Journal of Microbiological Methods
Volume	75
Issue number	1
Early online date	14 May 2008
DOIs	https://doi.org/10.1016/j.mimet.2008.04.006
Publication status	Published - 01 Sept 2008
Externally published	Yes

Keywords

cryptosporidium
immunomagnetic separation
human faeces
analytical sensitivity

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10.1016/j.mimet.2008.04.006

https://www.scopus.com/inward/record.uri?eid=2-s2.0-48749132800&doi=10.1016%2fj.mimet.2008.04.006&partnerID=40&md5=bb47e4433c109f6290e3da80fc5823e0

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title = "Evaluation of a modified semi-automated immunomagnetic separation technique for the detection of Cryptosporidium oocysts in human faeces",

abstract = "Routine diagnosis of Cryptosporidium infection relies on the oocyst as the diagnostic target. To improve analytical sensitivity, we modified and evaluated an immunomagnetic separation procedure for Cryptosporidium oocysts applicable to formed, liquid or mucoid human faeces. The analytical sensitivity of the method is < 5 oocysts per gram, regardless of stool consistency. ",

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AU - Watkins, J.

AU - Chalmers, R. M.

N1 - cited By 16

PY - 2008/9/1

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N2 - Routine diagnosis of Cryptosporidium infection relies on the oocyst as the diagnostic target. To improve analytical sensitivity, we modified and evaluated an immunomagnetic separation procedure for Cryptosporidium oocysts applicable to formed, liquid or mucoid human faeces. The analytical sensitivity of the method is < 5 oocysts per gram, regardless of stool consistency.

AB - Routine diagnosis of Cryptosporidium infection relies on the oocyst as the diagnostic target. To improve analytical sensitivity, we modified and evaluated an immunomagnetic separation procedure for Cryptosporidium oocysts applicable to formed, liquid or mucoid human faeces. The analytical sensitivity of the method is < 5 oocysts per gram, regardless of stool consistency.

KW - cryptosporidium

KW - immunomagnetic separation

KW - human faeces

KW - analytical sensitivity

U2 - 10.1016/j.mimet.2008.04.006

DO - 10.1016/j.mimet.2008.04.006

M3 - Article

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JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

IS - 1

ER -

Evaluation of a modified semi-automated immunomagnetic separation technique for the detection of Cryptosporidium oocysts in human faeces

Abstract

Keywords

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