Spontaneous DNA insertions from supernumerary B chromosomes (Bs) into the standard A chromosome complement were detected in rye (Secale cereale L.), using fluorescent in situ hybridization (FISH) analysis with the D1100 B–specific sequence probe. The insertions were seen in individuals derived from plants possessing deleted Bs, characterized in this study by not having the B–specific sequences that are normally found at the distal part of the long arm of the standard rye B. This result supports the case for the spontaneous introgression of B–specific DNA into the A chromosome genome, and it indicates that ‘cross–talking’ between A and B chromosomes may occur in wild populations.
|Journal||Proceedings of the Royal Society B: Biological Sciences|
|Publication status||Published - Dec 2004|
- B chromosome insertions
- deleted Bs
- fluorescent in situ hybridization with D1100 B-specific sequence