Evidence for enhanced central memory priming by live Mycobacterium bovis BCG vaccine in comparison with killed BCG formulations

Adam O. Whelan*, D. Craig Wright, Mark A. Chambers, Mahavir Singh, R. Glyn Hewinson, H. Martin Vordermeier

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)

Abstract

Development of cattle vaccines against bovine tuberculosis is a GB research priority. Recently, it has been shown that formalin-killed Bacille Calmette-Guérin (BCG) delivered with the liposomal adjuvant NAX687 imparted significant protection against Mycobacterium bovis infection in the guinea pig aerosol infection model. Extending these studies, we inoculated calves with live BCG, formalin-killed BCG and formalin-killed BCG formulated in NAX687. Live and killed BCG vaccine formulations induced primary effector T-cell populations comparably, both killed BCG formulations also induced potent humoral immune responses. In contrast, live BCG generated enhanced central memory responses against the protective antigen Ag85A whilst killed BCG-induced such responses only poorly. However, the poor capacity of killed BCG to generate central memory could be partially overcome by formulation with NAX687. Measurement of central memory responses induced by TB vaccine candidates in cattle may provide a useful correlate of protection and warrants further investigation in challenge experiments.

Original languageEnglish
Pages (from-to)166-173
Number of pages8
JournalVaccine
Volume26
Issue number2
Early online date26 Nov 2007
DOIs
Publication statusPublished - 10 Jan 2008

Keywords

  • Adjuvant
  • BCG
  • Cattle
  • Mycobacterium bovis
  • Vaccine

Fingerprint

Dive into the research topics of 'Evidence for enhanced central memory priming by live Mycobacterium bovis BCG vaccine in comparison with killed BCG formulations'. Together they form a unique fingerprint.

Cite this