Explanatory optimization of protein mass spectrometry via genetic search

Royston Goodacre; S. Vaidyanathan; David Iain Broadhurst; Douglas B. Kell

doi:10.1021/ac034669a

Explanatory optimization of protein mass spectrometry via genetic search

Royston Goodacre, S. Vaidyanathan, David Iain Broadhurst, Douglas B. Kell

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

40 Citations (Scopus)

Abstract

Optimizing experimental conditions for the effective analysis of intact proteins by mass spectrometry is challenging, as many analytical factors influence the spectral quality, often in very different ways for different proteins and especially with complex protein mixtures. We show that genetic search methods are highly effective in this kind of optimization and that it was possible in 6 generations with a total of

Original language	English
Pages (from-to)	6679-6686
Number of pages	8
Journal	Analytical Chemistry
Volume	75
Issue number	3
DOIs	https://doi.org/10.1021/ac034669a
Publication status	Published - 25 Oct 2003

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http://hdl.handle.net/2160/4146

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Explanatory optimization of protein mass spectrometry via genetic search

Abstract

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