Exploiting plant somatic hybrids for physical mappings of ESTs

J. Wardrop, J. Fuller, Wayne Powell, G. C. Machray

Research output: Contribution to journalArticlepeer-review

18 Citations (SciVal)

Abstract

Methods are described for the optimisation of the generation of radiation hybrids suitable for physical mapping of a plant (barley) genome. A combination of PCR-based technologies, involving the use of whole genome, mixed primer and hemi-nested primer amplifications, can greatly extend their utility for the physical mapping of expressed sequence tags (ESTs). Using panels of hybrids and ESTs, donor DNA retention and individual marker retention frequencies for the expressed portion of the barley genome in the hybrids were estimated.
Original languageEnglish
Pages (from-to)343-348
Number of pages6
JournalTheoretical and Applied Genetics
Volume108
Issue number2
Early online date25 Sept 2003
DOIs
Publication statusPublished - 01 Jan 2004

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