Exploiting plant somatic hybrids for physical mappings of ESTs

J. Wardrop; J. Fuller; Wayne Powell; G. C. Machray

doi:10.1007/s00122-003-1434-6

Exploiting plant somatic hybrids for physical mappings of ESTs

J. Wardrop, J. Fuller, Wayne Powell, G. C. Machray

Department of Life Sciences

Scottish Crop Research Institute

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

Methods are described for the optimisation of the generation of radiation hybrids suitable for physical mapping of a plant (barley) genome. A combination of PCR-based technologies, involving the use of whole genome, mixed primer and hemi-nested primer amplifications, can greatly extend their utility for the physical mapping of expressed sequence tags (ESTs). Using panels of hybrids and ESTs, donor DNA retention and individual marker retention frequencies for the expressed portion of the barley genome in the hybrids were estimated.

Original language	English
Pages (from-to)	343-348
Number of pages	6
Journal	Theoretical and Applied Genetics
Volume	108
Issue number	2
Early online date	25 Sept 2003
DOIs	https://doi.org/10.1007/s00122-003-1434-6
Publication status	Published - 01 Jan 2004

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http://hdl.handle.net/2160/5794

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AB - Methods are described for the optimisation of the generation of radiation hybrids suitable for physical mapping of a plant (barley) genome. A combination of PCR-based technologies, involving the use of whole genome, mixed primer and hemi-nested primer amplifications, can greatly extend their utility for the physical mapping of expressed sequence tags (ESTs). Using panels of hybrids and ESTs, donor DNA retention and individual marker retention frequencies for the expressed portion of the barley genome in the hybrids were estimated.

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Exploiting plant somatic hybrids for physical mappings of ESTs

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