Expressed sequence tag-derived microsatellite markers of perennial ryegrass (Lolium perenne L.)

Bruno Studer, Torben Asp, Ursula Frei, Stephan Hentrup, Helena Meally, Aurélie Guillard, Susanne Barth, Hilde Muylle, Isabel Roldan-Ruiz, Philippe Barre, Carole Koning-Boucoiran, Gerda Uuenk-Stunnenberg, Oene Dolstra, L. Skot, K. P. Skot, L. B. Turner, M. O. Humphreys, Roland Kolliker, Niels Roulund, Klaus K. NielsenThomas Lübberstedt

Research output: Contribution to journalArticlepeer-review

28 Citations (SciVal)


An expressed sequence tag (EST) library of the key grassland species perennial ryegrass (Lolium perenne L.) has been exploited as a resource for microsatellite marker development. Out of 955 simple sequence repeat (SSR) containing ESTs, 744 were used for primer design. Primer amplification was tested in eight genotypes of L. perenne and L. multiflorum representing (grand-) parents of four mapping populations and resulted in 464 successfully amplified EST-SSRs. Three hundred and six primer pairs successfully amplified products in the mapping population VrnA derived from two of the eight genotypes included in the original screening and revealed SSR polymorphisms for 143 ESTs. Here, we report on 464 EST-derived SSR primer sequences of perennial ryegrass established in laboratory assays, providing a dedicated tool for marker assisted breeding and comparative mapping within and among forage and turf grasses.
Original languageEnglish
Pages (from-to)533-548
Number of pages16
JournalMolecular Breeding
Issue number4
Early online date08 Jan 2008
Publication statusPublished - 01 May 2008


  • comparative mapping
  • simple sequence repeat (SSR)
  • expressed sequence tag (EST)
  • ryegrass (Lolium spp.)


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