Expressed sequence tag-derived microsatellite markers of perennial ryegrass (Lolium perenne L.)

Bruno Studer; Torben Asp; Ursula Frei; Stephan Hentrup; Helena Meally; Aurélie Guillard; Susanne Barth; Hilde Muylle; Isabel Roldan-Ruiz; Philippe Barre; Carole Koning-Boucoiran; Gerda Uuenk-Stunnenberg; Oene Dolstra; L. Skot; K. P. Skot; L. B. Turner; M. O. Humphreys; Roland Kolliker; Niels Roulund; Klaus K. Nielsen; Thomas  Lübberstedt

doi:10.1007/s11032-007-9148-0

Expressed sequence tag-derived microsatellite markers of perennial ryegrass (Lolium perenne L.)

Bruno Studer, Torben Asp, Ursula Frei, Stephan Hentrup, Helena Meally, Aurélie Guillard, Susanne Barth, Hilde Muylle, Isabel Roldan-Ruiz, Philippe Barre, Carole Koning-Boucoiran, Gerda Uuenk-Stunnenberg, Oene Dolstra, L. Skot, K. P. Skot, L. B. Turner, M. O. Humphreys, Roland Kolliker, Niels Roulund, Klaus K. NielsenThomas Lübberstedt

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

Abstract

An expressed sequence tag (EST) library of the key grassland species perennial ryegrass (Lolium perenne L.) has been exploited as a resource for microsatellite marker development. Out of 955 simple sequence repeat (SSR) containing ESTs, 744 were used for primer design. Primer amplification was tested in eight genotypes of L. perenne and L. multiflorum representing (grand-) parents of four mapping populations and resulted in 464 successfully amplified EST-SSRs. Three hundred and six primer pairs successfully amplified products in the mapping population VrnA derived from two of the eight genotypes included in the original screening and revealed SSR polymorphisms for 143 ESTs. Here, we report on 464 EST-derived SSR primer sequences of perennial ryegrass established in laboratory assays, providing a dedicated tool for marker assisted breeding and comparative mapping within and among forage and turf grasses.

Original language	English
Pages (from-to)	533-548
Number of pages	16
Journal	Molecular Breeding
Volume	21
Issue number	4
Early online date	08 Jan 2008
DOIs	https://doi.org/10.1007/s11032-007-9148-0
Publication status	Published - 01 May 2008

Keywords

comparative mapping
simple sequence repeat (SSR)
expressed sequence tag (EST)
ryegrass (Lolium spp.)

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Studer, B., Asp, T., Frei, U., Hentrup, S., Meally, H., Guillard, A., Barth, S., Muylle, H., Roldan-Ruiz, I., Barre, P., Koning-Boucoiran, C., Uuenk-Stunnenberg, G., Dolstra, O., Skot, L., Skot, K. P., Turner, L. B., Humphreys, M. O., Kolliker, R., Roulund, N., ... Lübberstedt, T. (2008). Expressed sequence tag-derived microsatellite markers of perennial ryegrass (Lolium perenne L.). Molecular Breeding, 21(4), 533-548. https://doi.org/10.1007/s11032-007-9148-0

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title = "Expressed sequence tag-derived microsatellite markers of perennial ryegrass (Lolium perenne L.)",

abstract = "An expressed sequence tag (EST) library of the key grassland species perennial ryegrass (Lolium perenne L.) has been exploited as a resource for microsatellite marker development. Out of 955 simple sequence repeat (SSR) containing ESTs, 744 were used for primer design. Primer amplification was tested in eight genotypes of L. perenne and L. multiflorum representing (grand-) parents of four mapping populations and resulted in 464 successfully amplified EST-SSRs. Three hundred and six primer pairs successfully amplified products in the mapping population VrnA derived from two of the eight genotypes included in the original screening and revealed SSR polymorphisms for 143 ESTs. Here, we report on 464 EST-derived SSR primer sequences of perennial ryegrass established in laboratory assays, providing a dedicated tool for marker assisted breeding and comparative mapping within and among forage and turf grasses.",

keywords = "comparative mapping, simple sequence repeat (SSR), expressed sequence tag (EST), ryegrass (Lolium spp.)",

author = "Bruno Studer and Torben Asp and Ursula Frei and Stephan Hentrup and Helena Meally and Aur{\'e}lie Guillard and Susanne Barth and Hilde Muylle and Isabel Roldan-Ruiz and Philippe Barre and Carole Koning-Boucoiran and Gerda Uuenk-Stunnenberg and Oene Dolstra and L. Skot and Skot, {K. P.} and Turner, {L. B.} and Humphreys, {M. O.} and Roland Kolliker and Niels Roulund and Nielsen, {Klaus K.} and Thomas L{\"u}bberstedt",

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doi = "10.1007/s11032-007-9148-0",

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Studer, B, Asp, T, Frei, U, Hentrup, S, Meally, H, Guillard, A, Barth, S, Muylle, H, Roldan-Ruiz, I, Barre, P, Koning-Boucoiran, C, Uuenk-Stunnenberg, G, Dolstra, O, Skot, L, Skot, KP, Turner, LB, Humphreys, MO, Kolliker, R, Roulund, N, Nielsen, KK & Lübberstedt, T 2008, 'Expressed sequence tag-derived microsatellite markers of perennial ryegrass (Lolium perenne L.)', Molecular Breeding, vol. 21, no. 4, pp. 533-548. https://doi.org/10.1007/s11032-007-9148-0

TY - JOUR

T1 - Expressed sequence tag-derived microsatellite markers of perennial ryegrass (Lolium perenne L.)

AU - Studer, Bruno

AU - Asp, Torben

AU - Frei, Ursula

AU - Hentrup, Stephan

AU - Meally, Helena

AU - Guillard, Aurélie

AU - Barth, Susanne

AU - Muylle, Hilde

AU - Roldan-Ruiz, Isabel

AU - Barre, Philippe

AU - Koning-Boucoiran, Carole

AU - Uuenk-Stunnenberg, Gerda

AU - Dolstra, Oene

AU - Skot, L.

AU - Skot, K. P.

AU - Turner, L. B.

AU - Humphreys, M. O.

AU - Kolliker, Roland

AU - Roulund, Niels

AU - Nielsen, Klaus K.

AU - Lübberstedt, Thomas

N1 - Studer, B., Asp, T., Frei, U., Hentrup, S. Meally, H., Guillard, A., Barth, S., Muylle, H., Roldan-Ruiz, I., Barre, P., Koning-Boucoiran, C., Uuenk-Stunnenberg, G., Dolstra, O., Skot, L., Skot, K. P., Turner, L. B., Humphreys, M. O., Kolliker, R., Roulund, N., Nielsen, K. K., Lubberstedt, T. (2008). Expressed sequence tag-derived microsatellite markers of perennial ryegrass (Lolium perenne L.). Molecular Breeding, 21,(4), 533-548. Sponsorship: Directorate for Food, Fisheries and Agri Business (DFFE), Denmark. IMPF: 02.01 RONO: 1340 3002

PY - 2008/5/1

Y1 - 2008/5/1

N2 - An expressed sequence tag (EST) library of the key grassland species perennial ryegrass (Lolium perenne L.) has been exploited as a resource for microsatellite marker development. Out of 955 simple sequence repeat (SSR) containing ESTs, 744 were used for primer design. Primer amplification was tested in eight genotypes of L. perenne and L. multiflorum representing (grand-) parents of four mapping populations and resulted in 464 successfully amplified EST-SSRs. Three hundred and six primer pairs successfully amplified products in the mapping population VrnA derived from two of the eight genotypes included in the original screening and revealed SSR polymorphisms for 143 ESTs. Here, we report on 464 EST-derived SSR primer sequences of perennial ryegrass established in laboratory assays, providing a dedicated tool for marker assisted breeding and comparative mapping within and among forage and turf grasses.

AB - An expressed sequence tag (EST) library of the key grassland species perennial ryegrass (Lolium perenne L.) has been exploited as a resource for microsatellite marker development. Out of 955 simple sequence repeat (SSR) containing ESTs, 744 were used for primer design. Primer amplification was tested in eight genotypes of L. perenne and L. multiflorum representing (grand-) parents of four mapping populations and resulted in 464 successfully amplified EST-SSRs. Three hundred and six primer pairs successfully amplified products in the mapping population VrnA derived from two of the eight genotypes included in the original screening and revealed SSR polymorphisms for 143 ESTs. Here, we report on 464 EST-derived SSR primer sequences of perennial ryegrass established in laboratory assays, providing a dedicated tool for marker assisted breeding and comparative mapping within and among forage and turf grasses.

KW - comparative mapping

KW - simple sequence repeat (SSR)

KW - expressed sequence tag (EST)

KW - ryegrass (Lolium spp.)

U2 - 10.1007/s11032-007-9148-0

DO - 10.1007/s11032-007-9148-0

M3 - Article

SN - 1572-9788

VL - 21

SP - 533

EP - 548

JO - Molecular Breeding

JF - Molecular Breeding

IS - 4

ER -

Expressed sequence tag-derived microsatellite markers of perennial ryegrass (Lolium perenne L.)

Abstract

Keywords

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