Expression of recombinant fusion proteins in attenuated bacteria

C. Mohammed Anjam Khan (Inventor), Carlos Estenio Hormaeche (Inventor), Bernardo Villarreal-Ramos (Inventor), Steven Neville Chatfield (Inventor), Gordon Dougan (Inventor)

Research output: Patent


The invention provides a DNA molecule comprising a promoter sequence operably linked to a DNA sequence encoding first and second proteins linked by a hinge region wherein in that the promoter sequence can be one having activity which is induced in response to a change in the surrounding environment and the first protein can be Tetanus toxin C fragment or one or more epitopes thereof. The invention also provides intermediate molecules having a promoter operably linked to a DNA sequence encoding a first antigenic sequence and a hinge region, and at or adjacent the 3′-end thereof one or more restriction sites for the introduction of a second anti-genic sequence. In addition, the invention provides replicable expression vectors containing the DNA fusion proteins expressed therefrom, bacterial transformed with the vectors and the use of the bacteria, in vaccines.
Original languageEnglish
Patent numberWO1994003615A1
Priority date31 Jul 1992
Filing date30 Jul 1993
Publication statusPublished - 17 Feb 1994


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