Extensive gene content variation in the Brachypodium distachyon pan-genome correlates with population structure

Sean P. Gordon, Bruno Contreras-Moreira, Daniel P. Woods, David L. Des Marais, Diane Burges, Shengqiang Shu, Christoph Stritt, Anne C. Roulin, Wendy Schackwitz, Ludmila Tyler, Joel Martin, Anna Lipzen, Niklas Dochy, Jeremy Phillips, Kerrie Barry, Koen Geuten, Hikmet Budak, Thomas E. Jeunger, Richard Amasino, Ana L. CaicedoDavid Goodstein, Patrick Davidson, Luis Mur, Melania Figueroa, Michael Freeling, Pilar Catalán, John P. Vogel

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Abstract

While prokaryotic pan-genomes have been shown to contain many more genes than any individual organism, the prevalence and functional significance of differentially present genes in eukaryotes remains poorly understood. Whole-genome de novo assembly and annotation of 54 lines of the grass Brachypodium distachyon yield a pan-genome containing nearly twice the number of genes found in any individual genome. Genes present in all lines are enriched for essential biological functions, while genes present in only some lines are enriched for conditionally beneficial functions (e.g., defense and development), display faster evolutionary rates, lie closer to transposable elements and are less likely to be syntenic with orthologous genes in other grasses. Our data suggest that differentially present genes contribute substantially to phenotypic variation within a eukaryote species, these genes have a major influence in population genetics, and transposable elements play a key role in pan-genome evolution.
Original languageEnglish
Article number2184
Number of pages13
JournalNature Communications
Volume8
Issue numberN/A
DOIs
Publication statusPublished - 19 Dec 2017

Keywords

  • comparative genomics
  • plant genetics
  • structural variation

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