Extracellular cyclophilin A possesses chemotaxic activity in cattle

Satoru Takanashi, Tomonori Nochi, Miku Abe, Nanami Itaya, Megumi Urakawa, Katsuyoshi Sato, Zhuang Tao, Saori Uemura, Tomohito Hayashi, Yoshio Kiku, haruki kitazawa, Michael Rose, Kouichi Watanabe, Hisashi Aso

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Cyclophilin A (CyPA) was originally discovered in bovine thymocytes as a cytosolic binding protein of the immunosuppressive drug cyclosporine A. Recent studies have revealed that in mice and humans, CyPA is secreted from cells in injured or infected tissues and plays a role in recruiting inflammatory cells in those tissues. Here we found that in cattle abundant level of extracellular CyPA was observed in tissues with inflammation. To aid in investigating the role of extracellular CyPA in cattle, we generated recombinant bovine CyPA (rbCyPA) and tested its biological activity as an inflammatory mediator. When bovine peripheral blood cells were treated with rbCyPA in vitro, we observed that rbCyPA reacts with the membranous surface of granulocytes, monocytes and lymphocytes. Chemotaxis analysis showed that the granulocytes migrate toward rbCyPA and the migration is inhibited by pre-treatment with an anti-bovine CyPA antibody. These results indicate that, as for mice and humans, extracellular CyPA possesses chemotactic activity to recruit inflammatory cells (e.g., granulocytes) in cattle, and could thus be a potential therapeutic target for the treatment of inflammation.
Original languageEnglish
Number of pages10
JournalVeterinary Research
Issue number80
Publication statusPublished - 11 Jul 2015


  • oxidative stress
  • host-response
  • inflammation
  • infections
  • strategies
  • antibodies
  • chemokines
  • leukemia
  • disease


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