Extracellular polysaccharide (EPS) production by a novel strain of yeast-like fungus Aureobasidium pullulans

Sreenivas Rao Ravella; Teresa Suarez Quinones; Andy Retter; Monika Heiermann; Thomas Amon; Phil J. Hobbs

doi:10.1016/j.carbpol.2010.05.039

Extracellular polysaccharide (EPS) production by a novel strain of yeast-like fungus Aureobasidium pullulans

Sreenivas Rao Ravella, Teresa Suarez Quinones, Andy Retter, Monika Heiermann, Thomas Amon, Phil J. Hobbs^*

^*Corresponding author for this work

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

68 Citations (Scopus)

Abstract

A yeast-like fungus producing a large amount of extracellular polysaccharide was isolated from the biogas reactors at North Wyke Research, UK (latitude, 50 degrees 46'29 '' N; longitude, 35 degrees 5'23 '' W). This strain was identified as Aureobasidium pullulans based on nucleotide sequence of the D1/D2 domain of the large-subunit (LSU) rRNA gene. Extracellular polysaccharide or pullulan, produced from this new strain of A. pullulans in shake flasks and a stirred tank fermenter was investigated. A maximum polysaccharide concentration of 40.1 gl(-1), and productivity of 12.5 gl(-1) per day was obtained in a batch culture from stirred tank fermenter. The optimal cultivation conditions for pullulan production for this strain in 250 ml shake flask containing 100 ml of sucrose medium were observed at 28 degrees C and with 200 rpm. The effect of different sugars and nitrogen sources on pullulan production was investigated. It was found that more pullulan was produced when the strain was grown in the medium supplemented with NaNO(3) than when it was cultivated in the medium supplemented with C5 and C6 sugars and other nitrogen sources. (C) 2010 Elsevier Ltd. All rights reserved.

Original language	English
Pages (from-to)	728-732
Number of pages	5
Journal	Carbohydrate Polymers
Volume	82
Issue number	3
DOIs	https://doi.org/10.1016/j.carbpol.2010.05.039
Publication status	Published - 15 Oct 2010

Keywords

EXOPOLYSACCHARIDES
Aureobasidium pullulans
STIRRED-TANK REACTOR
FB-1
Yeast-like fungus
Extracellular polysaccharide
BEET MOLASSES
Y68
MELANIN
DIGESTER
COLOR VARIANT STRAIN
Biogas reactor
D1/D2 domain
FERMENTATION

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title = "Extracellular polysaccharide (EPS) production by a novel strain of yeast-like fungus Aureobasidium pullulans",

abstract = "A yeast-like fungus producing a large amount of extracellular polysaccharide was isolated from the biogas reactors at North Wyke Research, UK (latitude, 50 degrees 46'29 '' N; longitude, 35 degrees 5'23 '' W). This strain was identified as Aureobasidium pullulans based on nucleotide sequence of the D1/D2 domain of the large-subunit (LSU) rRNA gene. Extracellular polysaccharide or pullulan, produced from this new strain of A. pullulans in shake flasks and a stirred tank fermenter was investigated. A maximum polysaccharide concentration of 40.1 gl(-1), and productivity of 12.5 gl(-1) per day was obtained in a batch culture from stirred tank fermenter. The optimal cultivation conditions for pullulan production for this strain in 250 ml shake flask containing 100 ml of sucrose medium were observed at 28 degrees C and with 200 rpm. The effect of different sugars and nitrogen sources on pullulan production was investigated. It was found that more pullulan was produced when the strain was grown in the medium supplemented with NaNO(3) than when it was cultivated in the medium supplemented with C5 and C6 sugars and other nitrogen sources. (C) 2010 Elsevier Ltd. All rights reserved.",

keywords = "EXOPOLYSACCHARIDES, Aureobasidium pullulans, STIRRED-TANK REACTOR, FB-1, Yeast-like fungus, Extracellular polysaccharide, BEET MOLASSES, Y68, MELANIN, DIGESTER, COLOR VARIANT STRAIN, Biogas reactor, D1/D2 domain, FERMENTATION",

author = "Ravella, {Sreenivas Rao} and Quinones, {Teresa Suarez} and Andy Retter and Monika Heiermann and Thomas Amon and Hobbs, {Phil J.}",

year = "2010",

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doi = "10.1016/j.carbpol.2010.05.039",

language = "English",

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journal = "Carbohydrate Polymers",

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T1 - Extracellular polysaccharide (EPS) production by a novel strain of yeast-like fungus Aureobasidium pullulans

AU - Ravella, Sreenivas Rao

AU - Quinones, Teresa Suarez

AU - Retter, Andy

AU - Heiermann, Monika

AU - Amon, Thomas

AU - Hobbs, Phil J.

PY - 2010/10/15

Y1 - 2010/10/15

N2 - A yeast-like fungus producing a large amount of extracellular polysaccharide was isolated from the biogas reactors at North Wyke Research, UK (latitude, 50 degrees 46'29 '' N; longitude, 35 degrees 5'23 '' W). This strain was identified as Aureobasidium pullulans based on nucleotide sequence of the D1/D2 domain of the large-subunit (LSU) rRNA gene. Extracellular polysaccharide or pullulan, produced from this new strain of A. pullulans in shake flasks and a stirred tank fermenter was investigated. A maximum polysaccharide concentration of 40.1 gl(-1), and productivity of 12.5 gl(-1) per day was obtained in a batch culture from stirred tank fermenter. The optimal cultivation conditions for pullulan production for this strain in 250 ml shake flask containing 100 ml of sucrose medium were observed at 28 degrees C and with 200 rpm. The effect of different sugars and nitrogen sources on pullulan production was investigated. It was found that more pullulan was produced when the strain was grown in the medium supplemented with NaNO(3) than when it was cultivated in the medium supplemented with C5 and C6 sugars and other nitrogen sources. (C) 2010 Elsevier Ltd. All rights reserved.

AB - A yeast-like fungus producing a large amount of extracellular polysaccharide was isolated from the biogas reactors at North Wyke Research, UK (latitude, 50 degrees 46'29 '' N; longitude, 35 degrees 5'23 '' W). This strain was identified as Aureobasidium pullulans based on nucleotide sequence of the D1/D2 domain of the large-subunit (LSU) rRNA gene. Extracellular polysaccharide or pullulan, produced from this new strain of A. pullulans in shake flasks and a stirred tank fermenter was investigated. A maximum polysaccharide concentration of 40.1 gl(-1), and productivity of 12.5 gl(-1) per day was obtained in a batch culture from stirred tank fermenter. The optimal cultivation conditions for pullulan production for this strain in 250 ml shake flask containing 100 ml of sucrose medium were observed at 28 degrees C and with 200 rpm. The effect of different sugars and nitrogen sources on pullulan production was investigated. It was found that more pullulan was produced when the strain was grown in the medium supplemented with NaNO(3) than when it was cultivated in the medium supplemented with C5 and C6 sugars and other nitrogen sources. (C) 2010 Elsevier Ltd. All rights reserved.

KW - EXOPOLYSACCHARIDES

KW - Aureobasidium pullulans

KW - STIRRED-TANK REACTOR

KW - FB-1

KW - Yeast-like fungus

KW - Extracellular polysaccharide

KW - BEET MOLASSES

KW - Y68

KW - MELANIN

KW - DIGESTER

KW - COLOR VARIANT STRAIN

KW - Biogas reactor

KW - D1/D2 domain

KW - FERMENTATION

UR - http://hdl.handle.net/2160/9089

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DO - 10.1016/j.carbpol.2010.05.039

M3 - Article

SN - 0144-8617

VL - 82

SP - 728

EP - 732

JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

IS - 3

ER -

Extracellular polysaccharide (EPS) production by a novel strain of yeast-like fungus Aureobasidium pullulans

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Keywords

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