Extracellular polysaccharide (EPS) production by a novel strain of yeast-like fungus Aureobasidium pullulans

Sreenivas Rao Ravella, Teresa Suarez Quinones, Andy Retter, Monika Heiermann, Thomas Amon, Phil J. Hobbs*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

62 Citations (SciVal)

Abstract

A yeast-like fungus producing a large amount of extracellular polysaccharide was isolated from the biogas reactors at North Wyke Research, UK (latitude, 50 degrees 46'29 '' N; longitude, 35 degrees 5'23 '' W). This strain was identified as Aureobasidium pullulans based on nucleotide sequence of the D1/D2 domain of the large-subunit (LSU) rRNA gene. Extracellular polysaccharide or pullulan, produced from this new strain of A. pullulans in shake flasks and a stirred tank fermenter was investigated. A maximum polysaccharide concentration of 40.1 gl(-1), and productivity of 12.5 gl(-1) per day was obtained in a batch culture from stirred tank fermenter. The optimal cultivation conditions for pullulan production for this strain in 250 ml shake flask containing 100 ml of sucrose medium were observed at 28 degrees C and with 200 rpm. The effect of different sugars and nitrogen sources on pullulan production was investigated. It was found that more pullulan was produced when the strain was grown in the medium supplemented with NaNO(3) than when it was cultivated in the medium supplemented with C5 and C6 sugars and other nitrogen sources. (C) 2010 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)728-732
Number of pages5
JournalCarbohydrate Polymers
Volume82
Issue number3
DOIs
Publication statusPublished - 15 Oct 2010

Keywords

  • EXOPOLYSACCHARIDES
  • Aureobasidium pullulans
  • STIRRED-TANK REACTOR
  • FB-1
  • Yeast-like fungus
  • Extracellular polysaccharide
  • BEET MOLASSES
  • Y68
  • MELANIN
  • DIGESTER
  • COLOR VARIANT STRAIN
  • Biogas reactor
  • D1/D2 domain
  • FERMENTATION

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